Histochemical methods for the demonstration of thomsen-friedenreich antigen in cell suspensions and tissue sections View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1978-08

AUTHORS

P. J. Klein, R. A. Newman, P. Müller, G. Uhlenbruck, H. E. Schaefer, K. J. Lennartz, R. Fischer

ABSTRACT

Summary1. Three different methods are described for the visualisation of the Thomsen-Friedenreich (TF) antigen on cell suspensions, formalin-fixed and paraffin embedded or frozen tissue sections:Rosette formation with chicken and sheep erythrocytes.Fluorescence-microscopy with fluorescein labelled peanut agglutinin.Autoradiography with3H-labelled peanut agglutinin.2. The TF antigen was shown, as far as presently investigated, to be exposed on various blood cells, glomerula of the kidney and normal mammary gland after neuraminidase treatment. Mammary gland was also shown to possess TF receptors without prior treatment with neuraminidase.3. The exposure of this cryptantigen can be brought about by bacterial or viral neuraminidase and is followed by an antigen/antibody reaction, which can lead to possible pathological consequences. More... »

PAGES

761-765

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01476765

DOI

http://dx.doi.org/10.1007/bf01476765

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044877040

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/682531


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