Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-04

AUTHORS

Dennis Brown, John Lydon, Margaret McLaughlin, Alan Stuart-Tilley, Robert Tyszkowski, Seth Alper

ABSTRACT

A simple method for antigen retrieval in tissue sections and cell cultures is described. Because many antibodies recognize denatured proteins on western blots, but are poorly reactive by immunocytochemistry, the effect of applying sodium dodecyl sulfate (SDS) to cryostat sections of tissues and to cell cultures prior to immunostaining was examined. In many cases, a 5-min pretreatment with 1% SDS produced a dramatic increase in staining intensity by indirect immunofluorescence. Among the antibodies tested that showed a positive effect of SDS were an anti-Na/K-ATPase monoclonal antibody, an anti-AE1/2 anion exchanger polyclonal antipeptide antibody, a monoclonal anti-caveolin antibody, and an anti-rab4 monoclonal antibody. In other cases, including antibodies against gp330, aquaporin 1, and aquaporin 2, no effect of SDS was detected. The results show that SDS treatment can be used as a simple method of antigen retrieval in cryostat sections and on cultured cells. In some cases, antigens were not detectable without pretreatment with SDS. More... »

PAGES

261-267

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01463929

DOI

http://dx.doi.org/10.1007/bf01463929

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035381246

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9072183


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