A host-cell-selected variant of influenza B virus with a single nucleotide substitution in HA affecting a potential glycosylation site was ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-03

AUTHORS

J. S. Oxford, G. C. Schild, T. Corcoran, R. Newman, D. Major, J. Robertson, J. Bootman, P. Higgins, W. Al-Nakib, D. A. J. Tyrrell

ABSTRACT

An influenza B virus was passaged in man (virus A) and then in human embryo trachea (C) and into embryonated eggs (D) or directly into eggs (B). Virus A, B, and C had the same (cell-like) haemagglutinin phenotype on reaction with selected monoclonal antibodies while D had an “egg-like” phenotype. The viruses were administered at a dose of 1,000 TCD50 (for MDCK cells) by intranasal inoculation to groups of 27 or 28 volunteers. Viruses A, B, and C all produced disease in six to eight volunteers, whereas D produced no illness and only four volunteers were infected. The viruses shed by the volunteers were indistinguishable from those with which they were inoculated. The haemagglutinin genes of the viruses were sequenced and changes were detected indicating amino acid substitutions at position 196–198 in the attenuated egg-grown virus D whereby a potential glycosylation site present in the other viruses was lost. More... »

PAGES

37-46

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01310701

DOI

http://dx.doi.org/10.1007/bf01310701

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003883828

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2310304


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