Further studies on plantlet production from cultured tissues ofSorghum bicolor View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-12

AUTHORS

D. I. Dunstan, K. C. Short, H. Dhaliwal, E. Thomas

ABSTRACT

Cultures capable of continuous plantlet production have been established from excised, immature embryos ofSorghum bicolor and the course of development of the plantlets has been followed by light and scanning electron microscopy. Such analyzes revealed that there are two distinct methods of plantlet production. Shoot primordia and embryo-like structures arisede novo from cells of the scutellum. However, when these cultures are transferred to fresh medium a further proliferation of shoot buds occurs by the formation of axillary shoot primordia. The cultures are therefore more comparable to shoot cultures than to callus cultures. Over 300 plantlets producedin vitro have been transferred to potting compost and grown to maturity. Most plants flowered and set seed. Fifteen plants were sterile but were of normal chromosome number (2 n=20) and it is presumed that the sterility was due to segregation of restorer genes in the immature embryos used to initiate some of the cultures. More... »

PAGES

355-361

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01276974

DOI

http://dx.doi.org/10.1007/bf01276974

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041595138


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