Chromosome number and the degree of cytokinin habituation of cultured tobacco pith cells View Full Text


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Article Info

DATE

1980-06

AUTHORS

A. N. Binns, F. Meins

ABSTRACT

Pith cells ofNicotiana tabacum L. cv. “Havana 425” gradually increase in degree of cytokinin habituation when serially propagated in culture. We tested the hypothesis that this variation results from changes in chromosome number. This involved inducing primary expiants of pith tissue to habituate and then analyzing clones derived from the tissues for mean chromosome number and relative growth rates on media with and without the cytokinin, kinetin. The experimental results did not support the working hypothesis. There was no conspicuous correlation between degree of habituation and chromosome number. Chromosome number, karyotypic stability and the capacity of cells for proliferation depended on the manner in which cultures had been initiated. Of particular interest was the observation that clones were predominantly diploid when isolated from pith tissues incubated on an auxincontaining medium at 35 °C. More... »

PAGES

179-187

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01276675

DOI

http://dx.doi.org/10.1007/bf01276675

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011609483


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