The development of double-seeded and mixed cell culture systems for use in diagnostic virology View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-09

AUTHORS

T. P. Subrahmanyan, P. Blaskovic, Ilene J. Wilson, N. A. Labzoffsky

ABSTRACT

Monkey kidney cells and human diploid cells were grown in a single culture tube. It was possible to maintain two distinct cell sheets on opposite walls of the culture tube or to mix the two cell suspensions and plant them together. The type and amount of medium required for the satisfactory growth and maintenance of these cultures appeared to depend on the total number of cells in the culture, glucose-free L-15 medium of Liebovitz being essential only in the case of the double-seeded cultures containing twice the usual number of cells. The double-seeded and mixed cell cultures were almost as sensitive as single seeded cultures in the case of several viruses. The use of these cultures for virus isolation from clinical specimens appears to be practical and economical. More... »

PAGES

291-297

Journal

TITLE

Archives of Virology

ISSUE

3

VOLUME

44

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01240617

DOI

http://dx.doi.org/10.1007/bf01240617

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045865518

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4365901


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