Ontology type: schema:ScholarlyArticle
1974-09
AUTHORST. P. Subrahmanyan, P. Blaskovic, Ilene J. Wilson, N. A. Labzoffsky
ABSTRACTMonkey kidney cells and human diploid cells were grown in a single culture tube. It was possible to maintain two distinct cell sheets on opposite walls of the culture tube or to mix the two cell suspensions and plant them together. The type and amount of medium required for the satisfactory growth and maintenance of these cultures appeared to depend on the total number of cells in the culture, glucose-free L-15 medium of Liebovitz being essential only in the case of the double-seeded cultures containing twice the usual number of cells. The double-seeded and mixed cell cultures were almost as sensitive as single seeded cultures in the case of several viruses. The use of these cultures for virus isolation from clinical specimens appears to be practical and economical. More... »
PAGES291-297
http://scigraph.springernature.com/pub.10.1007/bf01240617
DOIhttp://dx.doi.org/10.1007/bf01240617
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/4365901
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