Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-07

AUTHORS

Rodney S. Nairn, Gerald M. Adair, Thomas Porter, Sandra L. Pennington, Debra G. Smith, John H. Wilson, Michael M. Seidman

ABSTRACT

A 21-bp deletion in the third exon of the APRT gene in Chinese hamster ovary (CHO) cells was corrected by transfection with a plasmid containing hamster APRT sequences. Targeted correction frequencies in the range of 0.3-3.0 x 10(-6) were obtained with a vector containing 3.2 kb of APRT sequence homology. To examine the influence of vector configuration on targeted gene correction, a double-strand break was introduced at one of two positions in the vector prior to transfection by calcium phosphate-DNA coprecipitation or electroporation. A double-strand break in the region of APRT homology contained in the vector produced an insertion-type vector, while placement of the break just outside the region of homology produced a replacement-type vector. Gene targeting with both linear vector configurations yielded equivalent ratios of targeted recombinants to nontargeted vector integrants; however, targeting with the two different vector configurations resulted in different distributions of targeted recombination products. Analysis of 66 independent APRT+ recombinant clones by Southern hybridization showed that targeting with the vector in a replacement-type configuration yielded fewer targeted integrants and more target gene convertants than did the integration vector configuration. Targeted recombination was about fivefold more efficient with electroporation than with calcium phosphate-DNA coprecipitation; however, both gene transfer methods produced similar distributions of targeted recombinants, which depended only on targeting vector configuration. Our results demonstrate that insertion-type and replacement-type gene targeting vectors produce similar overall targeting frequencies in gene correction experiments, but that vector configuration can significantly influence the yield of particular recombinant types. More... »

PAGES

363-375

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01232748

DOI

http://dx.doi.org/10.1007/bf01232748

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046060918

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8105543


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenine Phosphoribosyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CHO Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetinae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Transfer Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymorphism, Restriction Fragment Length", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Nucleic Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "The University of Texas MD Anderson Cancer Center", 
          "id": "https://www.grid.ac/institutes/grid.240145.6", 
          "name": [
            "Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, 78957, Smithville, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nairn", 
        "givenName": "Rodney S.", 
        "id": "sg:person.0776426677.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776426677.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas MD Anderson Cancer Center", 
          "id": "https://www.grid.ac/institutes/grid.240145.6", 
          "name": [
            "Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, 78957, Smithville, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Adair", 
        "givenName": "Gerald M.", 
        "id": "sg:person.0764432405.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764432405.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Baylor College of Medicine", 
          "id": "https://www.grid.ac/institutes/grid.39382.33", 
          "name": [
            "Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 77030, Houston, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Porter", 
        "givenName": "Thomas", 
        "id": "sg:person.01111541210.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111541210.92"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Baylor College of Medicine", 
          "id": "https://www.grid.ac/institutes/grid.39382.33", 
          "name": [
            "Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 77030, Houston, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pennington", 
        "givenName": "Sandra L.", 
        "id": "sg:person.01157654410.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157654410.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The University of Texas MD Anderson Cancer Center", 
          "id": "https://www.grid.ac/institutes/grid.240145.6", 
          "name": [
            "Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, 78957, Smithville, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Smith", 
        "givenName": "Debra G.", 
        "id": "sg:person.01216537554.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216537554.31"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Baylor College of Medicine", 
          "id": "https://www.grid.ac/institutes/grid.39382.33", 
          "name": [
            "Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 77030, Houston, Texas"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wilson", 
        "givenName": "John H.", 
        "id": "sg:person.0772632074.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772632074.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Maryland Research Laboratories, Otsuka Pharmaceutical Co., Ltd., 20850, Rockville, Maryland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seidman", 
        "givenName": "Michael M.", 
        "id": "sg:person.01230543023.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230543023.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/350243a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002569490", 
          "https://doi.org/10.1038/350243a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.4.1.173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003156188"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.2.11.1353", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004396080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/330576a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004645846", 
          "https://doi.org/10.1038/330576a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/317230a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005647127", 
          "https://doi.org/10.1038/317230a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.12.8.3365", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006806233"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01543048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007976235", 
          "https://doi.org/10.1007/bf01543048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01543048", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007976235", 
          "https://doi.org/10.1007/bf01543048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.11.9.4509", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012315307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(83)90418-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012973560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.11.3.1402", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014754329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(83)90331-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016453858"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.3.941", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016510733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.75.3.1299", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020384020"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.5.4.684", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021927655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.81.10.3153", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023057225"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.4.2.157", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024182286"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/336348a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024391427", 
          "https://doi.org/10.1038/336348a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/09553008814550601", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028941254"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(75)90318-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029559011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2787(75)90318-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029559011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.12.4574", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030019294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.21.9498", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030422885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00332616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032668823", 
          "https://doi.org/10.1007/bf00332616"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.5.8.2080", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032928798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.80.19.5961", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033943349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/18.17.5173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035237119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/em.2860050206", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036599163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(85)90242-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036830078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.85.22.8583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037646266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(87)90646-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039136966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.1.227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042029467"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/mc.2940040616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042934265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.12.6.2464", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042946486"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.9.4.1621", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049277073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.78.4.2072", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051147601"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.24.8951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053251139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/mutage/4.5.349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059931011"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2660260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062549377"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2999980", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062580102"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1990.tb08260.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078435822"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078912805", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079419436", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-07", 
    "datePublishedReg": "1993-07-01", 
    "description": "A 21-bp deletion in the third exon of the APRT gene in Chinese hamster ovary (CHO) cells was corrected by transfection with a plasmid containing hamster APRT sequences. Targeted correction frequencies in the range of 0.3-3.0 x 10(-6) were obtained with a vector containing 3.2 kb of APRT sequence homology. To examine the influence of vector configuration on targeted gene correction, a double-strand break was introduced at one of two positions in the vector prior to transfection by calcium phosphate-DNA coprecipitation or electroporation. A double-strand break in the region of APRT homology contained in the vector produced an insertion-type vector, while placement of the break just outside the region of homology produced a replacement-type vector. Gene targeting with both linear vector configurations yielded equivalent ratios of targeted recombinants to nontargeted vector integrants; however, targeting with the two different vector configurations resulted in different distributions of targeted recombination products. Analysis of 66 independent APRT+ recombinant clones by Southern hybridization showed that targeting with the vector in a replacement-type configuration yielded fewer targeted integrants and more target gene convertants than did the integration vector configuration. Targeted recombination was about fivefold more efficient with electroporation than with calcium phosphate-DNA coprecipitation; however, both gene transfer methods produced similar distributions of targeted recombinants, which depended only on targeting vector configuration. Our results demonstrate that insertion-type and replacement-type gene targeting vectors produce similar overall targeting frequencies in gene correction experiments, but that vector configuration can significantly influence the yield of particular recombinant types.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf01232748", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2664476", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2466691", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2512070", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2467707", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1094443", 
        "issn": [
          "0740-7750", 
          "1572-9931"
        ], 
        "name": "Somatic Cell and Molecular Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "19"
      }
    ], 
    "name": "Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells", 
    "pagination": "363-375", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "db68c3654afcb845092a105ccaa8b1acaea14cd801f1c26f1303c65e6e09d737"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8105543"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8403568"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01232748"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046060918"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01232748", 
      "https://app.dimensions.ai/details/publication/pub.1046060918"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T00:12", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8695_00000496.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF01232748"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01232748'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01232748'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01232748'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01232748'


 

This table displays all metadata directly associated to this object as RDF triples.

311 TRIPLES      21 PREDICATES      85 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01232748 schema:about N071b260e328c45fd8097b4df6fad8c4c
2 N18fbc1d37b1241df98fe88ba7a4de740
3 N29dd0e6bf66345a1b7c41fe30551a487
4 N2aba058414c44a289fbd73a1819e2815
5 N4027f83effb148868f006dac29a0aad3
6 N5636f5847f6d46e4906c4bc942a050d4
7 N62aaa273ed644419b6da51be8cad518a
8 N69fa6872bc9944fcab665038b5dc3469
9 N7c8ac85aecb14d8591b9cb670dd3163e
10 N7f1b7ec97e524c4094df6558f1f6226c
11 N88314ab5c2b64e6291d4f2671852980d
12 N9a4daeab746e4fdead652cecd4664908
13 Nb7a5813808ac4ab5bbc67c08cd9af661
14 Nb7c23b0e275c4f6aae21496665e2d8a9
15 Nee29679f54bb4dbeb5e6b3c5f99d760a
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author Na5d5c36c0119484d9a8c06ef9d997963
19 schema:citation sg:pub.10.1007/bf00332616
20 sg:pub.10.1007/bf01543048
21 sg:pub.10.1038/317230a0
22 sg:pub.10.1038/330576a0
23 sg:pub.10.1038/336348a0
24 sg:pub.10.1038/350243a0
25 https://app.dimensions.ai/details/publication/pub.1078912805
26 https://app.dimensions.ai/details/publication/pub.1079419436
27 https://doi.org/10.1002/em.2860050206
28 https://doi.org/10.1002/j.1460-2075.1990.tb08260.x
29 https://doi.org/10.1002/mc.2940040616
30 https://doi.org/10.1016/0003-2697(83)90418-9
31 https://doi.org/10.1016/0005-2787(75)90318-4
32 https://doi.org/10.1016/0092-8674(83)90331-8
33 https://doi.org/10.1016/0092-8674(85)90242-9
34 https://doi.org/10.1016/0092-8674(87)90646-5
35 https://doi.org/10.1073/pnas.75.3.1299
36 https://doi.org/10.1073/pnas.78.4.2072
37 https://doi.org/10.1073/pnas.80.19.5961
38 https://doi.org/10.1073/pnas.81.10.3153
39 https://doi.org/10.1073/pnas.84.24.8951
40 https://doi.org/10.1073/pnas.85.22.8583
41 https://doi.org/10.1073/pnas.86.1.227
42 https://doi.org/10.1073/pnas.86.12.4574
43 https://doi.org/10.1073/pnas.88.21.9498
44 https://doi.org/10.1080/09553008814550601
45 https://doi.org/10.1093/mutage/4.5.349
46 https://doi.org/10.1093/nar/16.3.941
47 https://doi.org/10.1093/nar/18.17.5173
48 https://doi.org/10.1101/gad.2.11.1353
49 https://doi.org/10.1101/gad.4.2.157
50 https://doi.org/10.1126/science.2660260
51 https://doi.org/10.1126/science.2999980
52 https://doi.org/10.1128/mcb.11.3.1402
53 https://doi.org/10.1128/mcb.11.9.4509
54 https://doi.org/10.1128/mcb.12.6.2464
55 https://doi.org/10.1128/mcb.12.8.3365
56 https://doi.org/10.1128/mcb.4.1.173
57 https://doi.org/10.1128/mcb.5.4.684
58 https://doi.org/10.1128/mcb.5.8.2080
59 https://doi.org/10.1128/mcb.9.4.1621
60 schema:datePublished 1993-07
61 schema:datePublishedReg 1993-07-01
62 schema:description A 21-bp deletion in the third exon of the APRT gene in Chinese hamster ovary (CHO) cells was corrected by transfection with a plasmid containing hamster APRT sequences. Targeted correction frequencies in the range of 0.3-3.0 x 10(-6) were obtained with a vector containing 3.2 kb of APRT sequence homology. To examine the influence of vector configuration on targeted gene correction, a double-strand break was introduced at one of two positions in the vector prior to transfection by calcium phosphate-DNA coprecipitation or electroporation. A double-strand break in the region of APRT homology contained in the vector produced an insertion-type vector, while placement of the break just outside the region of homology produced a replacement-type vector. Gene targeting with both linear vector configurations yielded equivalent ratios of targeted recombinants to nontargeted vector integrants; however, targeting with the two different vector configurations resulted in different distributions of targeted recombination products. Analysis of 66 independent APRT+ recombinant clones by Southern hybridization showed that targeting with the vector in a replacement-type configuration yielded fewer targeted integrants and more target gene convertants than did the integration vector configuration. Targeted recombination was about fivefold more efficient with electroporation than with calcium phosphate-DNA coprecipitation; however, both gene transfer methods produced similar distributions of targeted recombinants, which depended only on targeting vector configuration. Our results demonstrate that insertion-type and replacement-type gene targeting vectors produce similar overall targeting frequencies in gene correction experiments, but that vector configuration can significantly influence the yield of particular recombinant types.
63 schema:genre research_article
64 schema:inLanguage en
65 schema:isAccessibleForFree false
66 schema:isPartOf N3ee5da1ef33e45a888a56725b4874d2e
67 N4260bf14ba2647a09f5d87ac0c450d7f
68 sg:journal.1094443
69 schema:name Targeting vector configuration and method of gene transfer influence targeted correction of theAPRT gene in Chinese hamster ovary cells
70 schema:pagination 363-375
71 schema:productId N28109334ef4a45afb5aea1e4ca462235
72 N3dfe46a4f6414fe3bf01231b724b04ce
73 Nadbd50832e314584bf780d064644854d
74 Ne263a9dceaf0479d8005b1c8bcbca9c2
75 Ne27e6231abef4ca3923e9db760632cb2
76 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046060918
77 https://doi.org/10.1007/bf01232748
78 schema:sdDatePublished 2019-04-11T00:12
79 schema:sdLicense https://scigraph.springernature.com/explorer/license/
80 schema:sdPublisher N33b2f1c9601245969a82f35490e6e244
81 schema:url http://link.springer.com/10.1007/BF01232748
82 sgo:license sg:explorer/license/
83 sgo:sdDataset articles
84 rdf:type schema:ScholarlyArticle
85 N071b260e328c45fd8097b4df6fad8c4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Plasmids
87 rdf:type schema:DefinedTerm
88 N18fbc1d37b1241df98fe88ba7a4de740 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Genetic Vectors
90 rdf:type schema:DefinedTerm
91 N28109334ef4a45afb5aea1e4ca462235 schema:name readcube_id
92 schema:value db68c3654afcb845092a105ccaa8b1acaea14cd801f1c26f1303c65e6e09d737
93 rdf:type schema:PropertyValue
94 N29dd0e6bf66345a1b7c41fe30551a487 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name CHO Cells
96 rdf:type schema:DefinedTerm
97 N2aba058414c44a289fbd73a1819e2815 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Animals
99 rdf:type schema:DefinedTerm
100 N33b2f1c9601245969a82f35490e6e244 schema:name Springer Nature - SN SciGraph project
101 rdf:type schema:Organization
102 N3dfe46a4f6414fe3bf01231b724b04ce schema:name doi
103 schema:value 10.1007/bf01232748
104 rdf:type schema:PropertyValue
105 N3ee5da1ef33e45a888a56725b4874d2e schema:issueNumber 4
106 rdf:type schema:PublicationIssue
107 N4027f83effb148868f006dac29a0aad3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name DNA
109 rdf:type schema:DefinedTerm
110 N4260bf14ba2647a09f5d87ac0c450d7f schema:volumeNumber 19
111 rdf:type schema:PublicationVolume
112 N5636f5847f6d46e4906c4bc942a050d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Adenine Phosphoribosyltransferase
114 rdf:type schema:DefinedTerm
115 N62aaa273ed644419b6da51be8cad518a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Gene Transfer Techniques
117 rdf:type schema:DefinedTerm
118 N69fa6872bc9944fcab665038b5dc3469 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Blotting, Southern
120 rdf:type schema:DefinedTerm
121 N754c163ae5cd42289740dedbb6f5057b rdf:first sg:person.01157654410.45
122 rdf:rest Ne1e536f366434380a90223a4c13315f1
123 N7c8ac85aecb14d8591b9cb670dd3163e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Base Sequence
125 rdf:type schema:DefinedTerm
126 N7f1b7ec97e524c4094df6558f1f6226c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Polymorphism, Restriction Fragment Length
128 rdf:type schema:DefinedTerm
129 N88314ab5c2b64e6291d4f2671852980d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Molecular Sequence Data
131 rdf:type schema:DefinedTerm
132 N88ee636ee4c940cfa1002c0116deaed3 rdf:first sg:person.0764432405.33
133 rdf:rest Nf29c1512daae4d86936b57f745d8a185
134 N9a4daeab746e4fdead652cecd4664908 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Cricetinae
136 rdf:type schema:DefinedTerm
137 N9e38bd283a144e23b894f46a9d6a37c0 schema:name Maryland Research Laboratories, Otsuka Pharmaceutical Co., Ltd., 20850, Rockville, Maryland
138 rdf:type schema:Organization
139 Na5d5c36c0119484d9a8c06ef9d997963 rdf:first sg:person.0776426677.90
140 rdf:rest N88ee636ee4c940cfa1002c0116deaed3
141 Nadbd50832e314584bf780d064644854d schema:name pubmed_id
142 schema:value 8105543
143 rdf:type schema:PropertyValue
144 Nb7a5813808ac4ab5bbc67c08cd9af661 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Sequence Homology, Nucleic Acid
146 rdf:type schema:DefinedTerm
147 Nb7c23b0e275c4f6aae21496665e2d8a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Recombination, Genetic
149 rdf:type schema:DefinedTerm
150 Ne1e536f366434380a90223a4c13315f1 rdf:first sg:person.01216537554.31
151 rdf:rest Ne22ed6ab28844949925b52f1b4a10fe5
152 Ne22ed6ab28844949925b52f1b4a10fe5 rdf:first sg:person.0772632074.07
153 rdf:rest Ne701234ec950440485606b0ad2f4f054
154 Ne263a9dceaf0479d8005b1c8bcbca9c2 schema:name nlm_unique_id
155 schema:value 8403568
156 rdf:type schema:PropertyValue
157 Ne27e6231abef4ca3923e9db760632cb2 schema:name dimensions_id
158 schema:value pub.1046060918
159 rdf:type schema:PropertyValue
160 Ne701234ec950440485606b0ad2f4f054 rdf:first sg:person.01230543023.58
161 rdf:rest rdf:nil
162 Nee29679f54bb4dbeb5e6b3c5f99d760a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Cell Line
164 rdf:type schema:DefinedTerm
165 Nf29c1512daae4d86936b57f745d8a185 rdf:first sg:person.01111541210.92
166 rdf:rest N754c163ae5cd42289740dedbb6f5057b
167 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
168 schema:name Biological Sciences
169 rdf:type schema:DefinedTerm
170 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
171 schema:name Genetics
172 rdf:type schema:DefinedTerm
173 sg:grant.2466691 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01232748
174 rdf:type schema:MonetaryGrant
175 sg:grant.2467707 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01232748
176 rdf:type schema:MonetaryGrant
177 sg:grant.2512070 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01232748
178 rdf:type schema:MonetaryGrant
179 sg:grant.2664476 http://pending.schema.org/fundedItem sg:pub.10.1007/bf01232748
180 rdf:type schema:MonetaryGrant
181 sg:journal.1094443 schema:issn 0740-7750
182 1572-9931
183 schema:name Somatic Cell and Molecular Genetics
184 rdf:type schema:Periodical
185 sg:person.01111541210.92 schema:affiliation https://www.grid.ac/institutes/grid.39382.33
186 schema:familyName Porter
187 schema:givenName Thomas
188 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111541210.92
189 rdf:type schema:Person
190 sg:person.01157654410.45 schema:affiliation https://www.grid.ac/institutes/grid.39382.33
191 schema:familyName Pennington
192 schema:givenName Sandra L.
193 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01157654410.45
194 rdf:type schema:Person
195 sg:person.01216537554.31 schema:affiliation https://www.grid.ac/institutes/grid.240145.6
196 schema:familyName Smith
197 schema:givenName Debra G.
198 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216537554.31
199 rdf:type schema:Person
200 sg:person.01230543023.58 schema:affiliation N9e38bd283a144e23b894f46a9d6a37c0
201 schema:familyName Seidman
202 schema:givenName Michael M.
203 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230543023.58
204 rdf:type schema:Person
205 sg:person.0764432405.33 schema:affiliation https://www.grid.ac/institutes/grid.240145.6
206 schema:familyName Adair
207 schema:givenName Gerald M.
208 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0764432405.33
209 rdf:type schema:Person
210 sg:person.0772632074.07 schema:affiliation https://www.grid.ac/institutes/grid.39382.33
211 schema:familyName Wilson
212 schema:givenName John H.
213 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772632074.07
214 rdf:type schema:Person
215 sg:person.0776426677.90 schema:affiliation https://www.grid.ac/institutes/grid.240145.6
216 schema:familyName Nairn
217 schema:givenName Rodney S.
218 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776426677.90
219 rdf:type schema:Person
220 sg:pub.10.1007/bf00332616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032668823
221 https://doi.org/10.1007/bf00332616
222 rdf:type schema:CreativeWork
223 sg:pub.10.1007/bf01543048 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007976235
224 https://doi.org/10.1007/bf01543048
225 rdf:type schema:CreativeWork
226 sg:pub.10.1038/317230a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005647127
227 https://doi.org/10.1038/317230a0
228 rdf:type schema:CreativeWork
229 sg:pub.10.1038/330576a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004645846
230 https://doi.org/10.1038/330576a0
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/336348a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024391427
233 https://doi.org/10.1038/336348a0
234 rdf:type schema:CreativeWork
235 sg:pub.10.1038/350243a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002569490
236 https://doi.org/10.1038/350243a0
237 rdf:type schema:CreativeWork
238 https://app.dimensions.ai/details/publication/pub.1078912805 schema:CreativeWork
239 https://app.dimensions.ai/details/publication/pub.1079419436 schema:CreativeWork
240 https://doi.org/10.1002/em.2860050206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036599163
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1002/j.1460-2075.1990.tb08260.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078435822
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1002/mc.2940040616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042934265
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/0003-2697(83)90418-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012973560
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/0005-2787(75)90318-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029559011
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/0092-8674(83)90331-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016453858
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/0092-8674(85)90242-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036830078
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/0092-8674(87)90646-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039136966
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1073/pnas.75.3.1299 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020384020
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1073/pnas.78.4.2072 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051147601
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1073/pnas.80.19.5961 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033943349
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1073/pnas.81.10.3153 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023057225
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1073/pnas.84.24.8951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053251139
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1073/pnas.85.22.8583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037646266
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1073/pnas.86.1.227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042029467
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1073/pnas.86.12.4574 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030019294
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1073/pnas.88.21.9498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030422885
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1080/09553008814550601 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028941254
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1093/mutage/4.5.349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059931011
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1093/nar/16.3.941 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016510733
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1093/nar/18.17.5173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035237119
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1101/gad.2.11.1353 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004396080
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1101/gad.4.2.157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024182286
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1126/science.2660260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062549377
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1126/science.2999980 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062580102
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1128/mcb.11.3.1402 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014754329
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1128/mcb.11.9.4509 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012315307
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1128/mcb.12.6.2464 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042946486
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1128/mcb.12.8.3365 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006806233
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1128/mcb.4.1.173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003156188
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1128/mcb.5.4.684 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021927655
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1128/mcb.5.8.2080 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032928798
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1128/mcb.9.4.1621 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049277073
305 rdf:type schema:CreativeWork
306 https://www.grid.ac/institutes/grid.240145.6 schema:alternateName The University of Texas MD Anderson Cancer Center
307 schema:name Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Science Park-Research Division, 78957, Smithville, Texas
308 rdf:type schema:Organization
309 https://www.grid.ac/institutes/grid.39382.33 schema:alternateName Baylor College of Medicine
310 schema:name Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, 77030, Houston, Texas
311 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...