The preparation of, and studies on, free cell suspensions from mouse pancreatic islets View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1974-10

AUTHORS

Å. Lernmark

ABSTRACT

Pancreatic islets, isolated from the pancreas of obese-hyperglycemic mice, were used to prepare free islet cells in suspension. Batches of 100 islets were disrupted by mechanical shaking for 10 sec in a Ca2+-free HEPES-buffered Krebs-Ring'er medium containing 1 mM EGTA. From 200–500 islets, about 2.2×106 cells could be obtained in suspension, corresponding to a yield of roughly 55% as calculated from the content of DNA in cells and islets. The isolated islet cells appeared well-preserved in the light and the electron microscope and seemed to exhibit a high degree of viability as judged by viable cell counts, a radioactive assay for lysis and insulin release. Insulin release from islet cell suspensions, as calculated per cell, per content of DNA or insulin or per packed cell volume, was stimulated by glucose alone or in combination with theophylline. The glucose response was low compared with that of intact isolated islets. More... »

PAGES

431-438

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01221634

DOI

http://dx.doi.org/10.1007/bf01221634

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027195140

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4375640


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