Bitter taste of enzymic hydrolysates of casein View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-07

AUTHORS

Elisabeth Bumberger, Hans-Dieter Belitz

ABSTRACT

beta-Casein A2 was isolated from milk of a homozygous cow and hydrolysed with trypsin. The hydrolysate was separated by RP-HPLC into 18 peptides, all but one of which could be attributed to the sequence of beta-casein on the basis of the amino acid composition. Some peptides overlapped. In total, they represented about 97% of the protein sequence. Only three peptides had a bitter taste, namely I49-N68 (recognition threshold 1.0 mg/ml, 0.45 mmol/l), I49-K97 (1.5 mg/ml, 0.28 mmol/l) and G203-V209 (0.175 mg/ml, 0.23 mmol/l). The contribution of the three peptides to the overall bitterness of the beta-casein hydrolysate (2.67 mg/ml) was about 11, 21, and 60%, respectively. Peptide I49-K97 was present in the hydrolysate together with its fragments I49-N68 and S69-K97. Remarkably, the smaller and more hydrophobic fragment I49-N68 was less bitter than I49-K97 on a molar basis, whereas the larger and more hydrophilic fragment S69-K97 had a neutral taste. These results show that in the case of larger peptides neither hydrophobicity nor size are responsible alone for bitter potency, but that conformational parameters must be of great importance. Furthermore, it can be concluded that only a part of the structure is responsible for the contact with the receptor. The bitterness of G203-V209 is discussed in connection with related synthetic peptides in the literature. More... »

PAGES

14-19

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01202693

DOI

http://dx.doi.org/10.1007/bf01202693

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006730104

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8356850


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Caseins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, High Pressure Liquid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Milk", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Structure-Activity Relationship", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Taste", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trypsin", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "German Research Centre for Food Chemistry", 
          "id": "https://www.grid.ac/institutes/grid.433727.6", 
          "name": [
            "Deutsche Forschungsanstalt f\u00fcr Lebensmittelchemie, Stiftung des \u00f6ffentlichen Rechts, M\u00fcnchen", 
            "Institut f\u00fcr Lebensmittelchemie der TU M\u00fcnchen, Lichtenbergstrasse 4, W-8046, Garching, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bumberger", 
        "givenName": "Elisabeth", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "German Research Centre for Food Chemistry", 
          "id": "https://www.grid.ac/institutes/grid.433727.6", 
          "name": [
            "Deutsche Forschungsanstalt f\u00fcr Lebensmittelchemie, Stiftung des \u00f6ffentlichen Rechts, M\u00fcnchen", 
            "Institut f\u00fcr Lebensmittelchemie der TU M\u00fcnchen, Lichtenbergstrasse 4, W-8046, Garching, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Belitz", 
        "givenName": "Hans-Dieter", 
        "id": "sg:person.013140335557.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013140335557.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1246/bcsj.56.1116", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000300362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001704966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jctb.280340311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001811677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jctb.280340311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001811677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb1961.38.375", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003995825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006655834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.53.2926", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007209202"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2621.1976.tb00590.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010780166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.90", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012929793"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/food.19750190508", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017798502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/87559128509540773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020468279"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.1163", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020642242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.97", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020679208"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jlac.197719770804", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021951217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1972.tb01722.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026609631"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1971.tb01590.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030041161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01869431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032038411", 
          "https://doi.org/10.1007/bf01869431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01869431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032038411", 
          "https://doi.org/10.1007/bf01869431"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/food.19750190507", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034551733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1972.tb01771.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035020705"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2621.1970.tb00940.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036593165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/food.19740180615", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037147943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/kagakutoseibutsu1962.20.709", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037632453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01106329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038689005", 
          "https://doi.org/10.1007/bf01106329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01106329", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038689005", 
          "https://doi.org/10.1007/bf01106329"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(72)90254-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041021137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2795(72)90254-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041021137"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.57.819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041759093"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0315-5463(82)72613-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043250740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1080/00021369.1974.10861162", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044040449"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01202798", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044218384", 
          "https://doi.org/10.1007/bf01202798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01202798", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044218384", 
          "https://doi.org/10.1007/bf01202798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01879606", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046894823", 
          "https://doi.org/10.1007/bf01879606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01879606", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046894823", 
          "https://doi.org/10.1007/bf01879606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb1961.49.2587", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047277204"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1271/bbb1961.36.1423", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048162760"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1246/bcsj.56.1678", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049809655"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3136/nskkk1962.28.6_325", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3136/nskkk1962.28.6_325", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050182949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0442.1982.tb01785.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053238530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1439-0442.1982.tb01785.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053238530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900019695", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053767761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900019695", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053767761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0022029900033112", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053936169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00881a009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055754695"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf60164a031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055920529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf60208a021", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055922944"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf60212a044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055923198"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1051/rnd:19740211", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057061096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/chemse/2.1.71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059459995"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081166633", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3168/jds.s0022-0302(79)83226-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086078141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3168/jds.s0022-0302(83)81851-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086086829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3168/jds.s0022-0302(90)78847-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086088214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bk-1979-0092.ch007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1089375913"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bk-1979-0115.ch004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1089376374"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-07", 
    "datePublishedReg": "1993-07-01", 
    "description": "beta-Casein A2 was isolated from milk of a homozygous cow and hydrolysed with trypsin. The hydrolysate was separated by RP-HPLC into 18 peptides, all but one of which could be attributed to the sequence of beta-casein on the basis of the amino acid composition. Some peptides overlapped. In total, they represented about 97% of the protein sequence. Only three peptides had a bitter taste, namely I49-N68 (recognition threshold 1.0 mg/ml, 0.45 mmol/l), I49-K97 (1.5 mg/ml, 0.28 mmol/l) and G203-V209 (0.175 mg/ml, 0.23 mmol/l). The contribution of the three peptides to the overall bitterness of the beta-casein hydrolysate (2.67 mg/ml) was about 11, 21, and 60%, respectively. Peptide I49-K97 was present in the hydrolysate together with its fragments I49-N68 and S69-K97. Remarkably, the smaller and more hydrophobic fragment I49-N68 was less bitter than I49-K97 on a molar basis, whereas the larger and more hydrophilic fragment S69-K97 had a neutral taste. These results show that in the case of larger peptides neither hydrophobicity nor size are responsible alone for bitter potency, but that conformational parameters must be of great importance. Furthermore, it can be concluded that only a part of the structure is responsible for the contact with the receptor. The bitterness of G203-V209 is discussed in connection with related synthetic peptides in the literature.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf01202693", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1312167", 
        "issn": [
          "0044-3026", 
          "1438-2385"
        ], 
        "name": "European Food Research and Technology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "197"
      }
    ], 
    "name": "Bitter taste of enzymic hydrolysates of casein", 
    "pagination": "14-19", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "200eab0194c788fc2cb6f8d608e82d6465764ba4123ccceaf8c4197192f80def"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8356850"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7509812"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf01202693"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006730104"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf01202693", 
      "https://app.dimensions.ai/details/publication/pub.1006730104"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:34", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46772_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF01202693"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01202693'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01202693'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01202693'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01202693'


 

This table displays all metadata directly associated to this object as RDF triples.

264 TRIPLES      21 PREDICATES      87 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf01202693 schema:about N172f196f626a4749b0546cdd39d98505
2 N37be909333c64312a31c1b13b5257668
3 N39a72fd994d34d319780cb5642f5db1f
4 N51041a511948477bb679bd3b9163ec47
5 N52c6d4e35a2d48b1848d5053bf97ebcb
6 N5517b4b53d964165a74bfa4c22dbea96
7 Nb19536eff26b4dfea3b9b7c5f26c3ee9
8 Nbed6a079f96f4b06ab6e00929d35fda6
9 Nee4b4d425abb4718992d802719ccedb9
10 Nfac1af0152934e128927f2c734954cd5
11 Nfc942f4bc52047d58078646361b0977f
12 anzsrc-for:06
13 anzsrc-for:0601
14 schema:author N86ae395458104bf0bf65cbc6597da82e
15 schema:citation sg:pub.10.1007/bf01106329
16 sg:pub.10.1007/bf01202798
17 sg:pub.10.1007/bf01869431
18 sg:pub.10.1007/bf01879606
19 https://app.dimensions.ai/details/publication/pub.1081166633
20 https://doi.org/10.1002/food.19740180615
21 https://doi.org/10.1002/food.19750190507
22 https://doi.org/10.1002/food.19750190508
23 https://doi.org/10.1002/jctb.280340311
24 https://doi.org/10.1002/jlac.197719770804
25 https://doi.org/10.1016/0005-2795(72)90254-1
26 https://doi.org/10.1016/s0315-5463(82)72613-6
27 https://doi.org/10.1017/s0022029900019695
28 https://doi.org/10.1017/s0022029900033112
29 https://doi.org/10.1021/bk-1979-0092.ch007
30 https://doi.org/10.1021/bk-1979-0115.ch004
31 https://doi.org/10.1021/ja00881a009
32 https://doi.org/10.1021/jf60164a031
33 https://doi.org/10.1021/jf60208a021
34 https://doi.org/10.1021/jf60212a044
35 https://doi.org/10.1051/rnd:19740211
36 https://doi.org/10.1080/00021369.1974.10861162
37 https://doi.org/10.1080/87559128509540773
38 https://doi.org/10.1093/chemse/2.1.71
39 https://doi.org/10.1111/j.1365-2621.1970.tb00940.x
40 https://doi.org/10.1111/j.1365-2621.1976.tb00590.x
41 https://doi.org/10.1111/j.1432-1033.1971.tb01590.x
42 https://doi.org/10.1111/j.1432-1033.1972.tb01722.x
43 https://doi.org/10.1111/j.1432-1033.1972.tb01771.x
44 https://doi.org/10.1111/j.1439-0442.1982.tb01785.x
45 https://doi.org/10.1246/bcsj.53.2926
46 https://doi.org/10.1246/bcsj.56.1116
47 https://doi.org/10.1246/bcsj.56.1678
48 https://doi.org/10.1246/bcsj.57.103
49 https://doi.org/10.1246/bcsj.57.1163
50 https://doi.org/10.1246/bcsj.57.301
51 https://doi.org/10.1246/bcsj.57.819
52 https://doi.org/10.1246/bcsj.57.90
53 https://doi.org/10.1246/bcsj.57.97
54 https://doi.org/10.1271/bbb1961.36.1423
55 https://doi.org/10.1271/bbb1961.38.375
56 https://doi.org/10.1271/bbb1961.49.2587
57 https://doi.org/10.1271/kagakutoseibutsu1962.20.709
58 https://doi.org/10.3136/nskkk1962.28.6_325
59 https://doi.org/10.3168/jds.s0022-0302(79)83226-9
60 https://doi.org/10.3168/jds.s0022-0302(83)81851-7
61 https://doi.org/10.3168/jds.s0022-0302(90)78847-9
62 schema:datePublished 1993-07
63 schema:datePublishedReg 1993-07-01
64 schema:description beta-Casein A2 was isolated from milk of a homozygous cow and hydrolysed with trypsin. The hydrolysate was separated by RP-HPLC into 18 peptides, all but one of which could be attributed to the sequence of beta-casein on the basis of the amino acid composition. Some peptides overlapped. In total, they represented about 97% of the protein sequence. Only three peptides had a bitter taste, namely I49-N68 (recognition threshold 1.0 mg/ml, 0.45 mmol/l), I49-K97 (1.5 mg/ml, 0.28 mmol/l) and G203-V209 (0.175 mg/ml, 0.23 mmol/l). The contribution of the three peptides to the overall bitterness of the beta-casein hydrolysate (2.67 mg/ml) was about 11, 21, and 60%, respectively. Peptide I49-K97 was present in the hydrolysate together with its fragments I49-N68 and S69-K97. Remarkably, the smaller and more hydrophobic fragment I49-N68 was less bitter than I49-K97 on a molar basis, whereas the larger and more hydrophilic fragment S69-K97 had a neutral taste. These results show that in the case of larger peptides neither hydrophobicity nor size are responsible alone for bitter potency, but that conformational parameters must be of great importance. Furthermore, it can be concluded that only a part of the structure is responsible for the contact with the receptor. The bitterness of G203-V209 is discussed in connection with related synthetic peptides in the literature.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree false
68 schema:isPartOf Na8bfe9a3fe6640fbb0402434b9037368
69 Ne73eda881fea42d19a11c13e0887b860
70 sg:journal.1312167
71 schema:name Bitter taste of enzymic hydrolysates of casein
72 schema:pagination 14-19
73 schema:productId N1ca50872d5a847309e319fd26f9478e3
74 N46793c24bed7494bb069be07b7a54c14
75 N9e012f8bbb5d4222a6f571667332fc1e
76 Nd227b49887d9405ca16390205e5fb4c7
77 Nf2fc46c9a466459b8bf59c4ec042cbcd
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006730104
79 https://doi.org/10.1007/bf01202693
80 schema:sdDatePublished 2019-04-11T13:34
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher N1700544368bf4375b80f03a4b9bc093d
83 schema:url http://link.springer.com/10.1007/BF01202693
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N1700544368bf4375b80f03a4b9bc093d schema:name Springer Nature - SN SciGraph project
88 rdf:type schema:Organization
89 N172f196f626a4749b0546cdd39d98505 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Caseins
91 rdf:type schema:DefinedTerm
92 N1ca50872d5a847309e319fd26f9478e3 schema:name doi
93 schema:value 10.1007/bf01202693
94 rdf:type schema:PropertyValue
95 N37be909333c64312a31c1b13b5257668 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Amino Acid Sequence
97 rdf:type schema:DefinedTerm
98 N39a72fd994d34d319780cb5642f5db1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Animals
100 rdf:type schema:DefinedTerm
101 N46793c24bed7494bb069be07b7a54c14 schema:name pubmed_id
102 schema:value 8356850
103 rdf:type schema:PropertyValue
104 N51041a511948477bb679bd3b9163ec47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Amino Acids
106 rdf:type schema:DefinedTerm
107 N52c6d4e35a2d48b1848d5053bf97ebcb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name Molecular Sequence Data
109 rdf:type schema:DefinedTerm
110 N5517b4b53d964165a74bfa4c22dbea96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Milk
112 rdf:type schema:DefinedTerm
113 N7237f3cc89594f009a62f333aa077617 rdf:first sg:person.013140335557.48
114 rdf:rest rdf:nil
115 N86ae395458104bf0bf65cbc6597da82e rdf:first Nd5197da78d674ab9a8885486f46277de
116 rdf:rest N7237f3cc89594f009a62f333aa077617
117 N9e012f8bbb5d4222a6f571667332fc1e schema:name readcube_id
118 schema:value 200eab0194c788fc2cb6f8d608e82d6465764ba4123ccceaf8c4197192f80def
119 rdf:type schema:PropertyValue
120 Na8bfe9a3fe6640fbb0402434b9037368 schema:issueNumber 1
121 rdf:type schema:PublicationIssue
122 Nb19536eff26b4dfea3b9b7c5f26c3ee9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Chromatography, High Pressure Liquid
124 rdf:type schema:DefinedTerm
125 Nbed6a079f96f4b06ab6e00929d35fda6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Trypsin
127 rdf:type schema:DefinedTerm
128 Nd227b49887d9405ca16390205e5fb4c7 schema:name dimensions_id
129 schema:value pub.1006730104
130 rdf:type schema:PropertyValue
131 Nd5197da78d674ab9a8885486f46277de schema:affiliation https://www.grid.ac/institutes/grid.433727.6
132 schema:familyName Bumberger
133 schema:givenName Elisabeth
134 rdf:type schema:Person
135 Ne73eda881fea42d19a11c13e0887b860 schema:volumeNumber 197
136 rdf:type schema:PublicationVolume
137 Nee4b4d425abb4718992d802719ccedb9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Humans
139 rdf:type schema:DefinedTerm
140 Nf2fc46c9a466459b8bf59c4ec042cbcd schema:name nlm_unique_id
141 schema:value 7509812
142 rdf:type schema:PropertyValue
143 Nfac1af0152934e128927f2c734954cd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Structure-Activity Relationship
145 rdf:type schema:DefinedTerm
146 Nfc942f4bc52047d58078646361b0977f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Taste
148 rdf:type schema:DefinedTerm
149 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
150 schema:name Biological Sciences
151 rdf:type schema:DefinedTerm
152 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biochemistry and Cell Biology
154 rdf:type schema:DefinedTerm
155 sg:journal.1312167 schema:issn 0044-3026
156 1438-2385
157 schema:name European Food Research and Technology
158 rdf:type schema:Periodical
159 sg:person.013140335557.48 schema:affiliation https://www.grid.ac/institutes/grid.433727.6
160 schema:familyName Belitz
161 schema:givenName Hans-Dieter
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013140335557.48
163 rdf:type schema:Person
164 sg:pub.10.1007/bf01106329 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038689005
165 https://doi.org/10.1007/bf01106329
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/bf01202798 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044218384
168 https://doi.org/10.1007/bf01202798
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/bf01869431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032038411
171 https://doi.org/10.1007/bf01869431
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/bf01879606 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046894823
174 https://doi.org/10.1007/bf01879606
175 rdf:type schema:CreativeWork
176 https://app.dimensions.ai/details/publication/pub.1081166633 schema:CreativeWork
177 https://doi.org/10.1002/food.19740180615 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037147943
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1002/food.19750190507 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034551733
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1002/food.19750190508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017798502
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1002/jctb.280340311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001811677
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1002/jlac.197719770804 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021951217
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/0005-2795(72)90254-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041021137
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/s0315-5463(82)72613-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043250740
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1017/s0022029900019695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053767761
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1017/s0022029900033112 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053936169
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1021/bk-1979-0092.ch007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089375913
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1021/bk-1979-0115.ch004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1089376374
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1021/ja00881a009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055754695
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1021/jf60164a031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055920529
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1021/jf60208a021 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055922944
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1021/jf60212a044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055923198
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1051/rnd:19740211 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057061096
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1080/00021369.1974.10861162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044040449
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1080/87559128509540773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020468279
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1093/chemse/2.1.71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059459995
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1111/j.1365-2621.1970.tb00940.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036593165
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1111/j.1365-2621.1976.tb00590.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010780166
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1111/j.1432-1033.1971.tb01590.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030041161
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1111/j.1432-1033.1972.tb01722.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026609631
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1111/j.1432-1033.1972.tb01771.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035020705
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1111/j.1439-0442.1982.tb01785.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053238530
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1246/bcsj.53.2926 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007209202
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1246/bcsj.56.1116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000300362
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1246/bcsj.56.1678 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049809655
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1246/bcsj.57.103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001704966
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1246/bcsj.57.1163 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020642242
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1246/bcsj.57.301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006655834
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1246/bcsj.57.819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041759093
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1246/bcsj.57.90 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012929793
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1246/bcsj.57.97 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020679208
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1271/bbb1961.36.1423 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048162760
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1271/bbb1961.38.375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003995825
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1271/bbb1961.49.2587 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047277204
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1271/kagakutoseibutsu1962.20.709 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037632453
252 rdf:type schema:CreativeWork
253 https://doi.org/10.3136/nskkk1962.28.6_325 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050182949
254 rdf:type schema:CreativeWork
255 https://doi.org/10.3168/jds.s0022-0302(79)83226-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086078141
256 rdf:type schema:CreativeWork
257 https://doi.org/10.3168/jds.s0022-0302(83)81851-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086086829
258 rdf:type schema:CreativeWork
259 https://doi.org/10.3168/jds.s0022-0302(90)78847-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086088214
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.433727.6 schema:alternateName German Research Centre for Food Chemistry
262 schema:name Deutsche Forschungsanstalt für Lebensmittelchemie, Stiftung des öffentlichen Rechts, München
263 Institut für Lebensmittelchemie der TU München, Lichtenbergstrasse 4, W-8046, Garching, Germany
264 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...