Reversed-phase HPLC analysis of peptides in standard and dairy samples using on-line absorbance and post-column OPA-fluorescence detection View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-10

AUTHORS

Tomás Herraiz, Vicente Casal, M. Carmen Polo

ABSTRACT

Absorbance and post-column o-phthalaldehyde (OPA)-fluorescent detection were used to analyse standard and dairy peptides following reverse-phase HPLC. Using both detection systems on-line provides additional information on the presence of peptides in dairy products. The detection response depends on the amino acid composition of the peptide involved. Among the peptides used, glutathione, lysine-containing peptides and peptides with glycine as the N-terminal residue give the highest fluorescence after the OPA post-column reaction. Absorbance is more sensitive than fluorescence for peptides with aromatic amino acids. Different parameters, such as the flow rate of OPA, the amount of mercaptoethanol in the OPA reagent and the temperature of reaction, substantially influence the fluorescent response of peptides. The interest of using on-line absorbance and fluorescence detection is highlighted by analysing peptides from skim milk and from a tryptic hydrolysate. More... »

PAGES

265-269

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01193309

DOI

http://dx.doi.org/10.1007/bf01193309

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038618652

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7839733


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