Inhibitory effect of GA3 on the development of thidiazuron-induced somatic embryogenesis in geranium (Pelargonium xhortorum Bailey) hypocotyl cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-03

AUTHORS

M. J. Hutchinson, S. KrishnaRaj, P. K. Saxena

ABSTRACT

Somatic embryogenesis in geranium (Pelargonium xhortorum Bailey cv 'Scarlet Orbit Improved') can be achieved by incubating hypocotyl explants on MS medium supplemented with thidiazuron (TDZ; 10 μM for 3 days followed by subculture on medium devoid of any plant growth regulators. The presence of gibberellins (GAs) during both the induction and expression phases of embryogenesis was significantly detrimental to somatic embryo formation on the hypocotyl explants. The addition of the GA-synthesis inhibitors paclobutrazol, uniconazole or ancymidol during the period of growth and differentiation of somatic embryos increased the number of somatic embryos formed on each explant. However, paclobutrazol added during the period of induction had no significant influence on somatic embryo formation. Results suggest that both exogenously supplied as well as endogenous GAs play a role, albeit a negative one, on somatic embryogenesis of geranium. More... »

PAGES

435-438

Journal

TITLE

Plant Cell Reports

ISSUE

6

VOLUME

16

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01146789

DOI

http://dx.doi.org/10.1007/bf01146789

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046776311

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30727657


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