A simple method for large-scale electrofusion and culture of plant protoplasts View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1984-04

AUTHORS

J. W. Watts, Janet M. King

ABSTRACT

A simple method is described for the electrofusion of plant protoplasts. Protoplasts were aggregated in a radio-frequency field (10 V RMS, 0.5 MHz) for 15-30 s with an inter-electrode distance of 5 mm. They were then fused with a 300-V DC pulse. The protoplasts were able to divide after this treatment. A transferrable electrode permitted electrofusion of 1-ml volumes of culture in standard tissue-culture dishes in about 20 s. More... »

PAGES

335-342

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01140497

DOI

http://dx.doi.org/10.1007/bf01140497

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030117333

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6733258


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