Covalent stabilization of alginate gel for the entrapment of living whole cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-08

AUTHORS

Staffan Birnbaum, Robert Pendleton, Per-Olof Larsson, Klaus Mosbach

ABSTRACT

Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients. The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized. More... »

PAGES

393-400

References to SciGraph publications

  • 1977-09. Screening of matrix suitable for immobilization of microbial cells in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1979-06. Steroid transformation by living cells immobilized in calcium alginate in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1980-12. Denitrification of water using immobilized Pseudomonas denitrificans cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1979-12. Alcohol production by magnetic immobilized yeast in BIOTECHNOLOGY LETTERS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01134097

    DOI

    http://dx.doi.org/10.1007/bf01134097

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034600063


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Lund University", 
              "id": "https://www.grid.ac/institutes/grid.4514.4", 
              "name": [
                "Pure and Applied Biochemistry Chemical Center, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Birnbaum", 
            "givenName": "Staffan", 
            "id": "sg:person.012720513767.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012720513767.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lund University", 
              "id": "https://www.grid.ac/institutes/grid.4514.4", 
              "name": [
                "Pure and Applied Biochemistry Chemical Center, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pendleton", 
            "givenName": "Robert", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lund University", 
              "id": "https://www.grid.ac/institutes/grid.4514.4", 
              "name": [
                "Pure and Applied Biochemistry Chemical Center, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Larsson", 
            "givenName": "Per-Olof", 
            "id": "sg:person.071444746.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.071444746.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Lund University", 
              "id": "https://www.grid.ac/institutes/grid.4514.4", 
              "name": [
                "Pure and Applied Biochemistry Chemical Center, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Mosbach", 
            "givenName": "Klaus", 
            "id": "sg:person.01362574601.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362574601.27"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/0014-5793(80)81238-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010371868"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01387977", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012803158", 
              "https://doi.org/10.1007/bf01387977"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00505015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015922032", 
              "https://doi.org/10.1007/bf00505015"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00505015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015922032", 
              "https://doi.org/10.1007/bf00505015"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(80)80463-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034460040"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260211202", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040101312"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02996744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044893386", 
              "https://doi.org/10.1007/bf02996744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02996744", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044893386", 
              "https://doi.org/10.1007/bf02996744"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.260190309", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047950144"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(80)90097-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051737539"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(80)90097-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051737539"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00498724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053723453", 
              "https://doi.org/10.1007/bf00498724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00498724", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053723453", 
              "https://doi.org/10.1007/bf00498724"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.6776628", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062640201"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a127107", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083613495"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1981-08", 
        "datePublishedReg": "1981-08-01", 
        "description": "Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients. The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf01134097", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1289037", 
            "issn": [
              "0951-208X", 
              "1573-6784"
            ], 
            "name": "Biotechnology Letters", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "8", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "3"
          }
        ], 
        "name": "Covalent stabilization of alginate gel for the entrapment of living whole cells", 
        "pagination": "393-400", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "a4d290222b9f360915221f9e00f57b41ea55b648f416638425ed78aff49428d3"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf01134097"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1034600063"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf01134097", 
          "https://app.dimensions.ai/details/publication/pub.1034600063"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:27", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000370_0000000370/records_46738_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF01134097"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf01134097'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf01134097'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf01134097'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf01134097'


     

    This table displays all metadata directly associated to this object as RDF triples.

    118 TRIPLES      21 PREDICATES      38 URIs      19 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf01134097 schema:about anzsrc-for:06
    2 anzsrc-for:0601
    3 schema:author Na548bda68e8243afb695362dd21104d1
    4 schema:citation sg:pub.10.1007/bf00498724
    5 sg:pub.10.1007/bf00505015
    6 sg:pub.10.1007/bf01387977
    7 sg:pub.10.1007/bf02996744
    8 https://doi.org/10.1002/bit.260190309
    9 https://doi.org/10.1002/bit.260211202
    10 https://doi.org/10.1016/0014-5793(80)80463-7
    11 https://doi.org/10.1016/0014-5793(80)81238-5
    12 https://doi.org/10.1016/0141-0229(80)90097-6
    13 https://doi.org/10.1093/oxfordjournals.jbchem.a127107
    14 https://doi.org/10.1126/science.6776628
    15 schema:datePublished 1981-08
    16 schema:datePublishedReg 1981-08-01
    17 schema:description Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients. The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized.
    18 schema:genre research_article
    19 schema:inLanguage en
    20 schema:isAccessibleForFree false
    21 schema:isPartOf N25e204012a7e4980889bb8cd7fd9cf5f
    22 Nf4e506f906704f518d2190d17a07767f
    23 sg:journal.1289037
    24 schema:name Covalent stabilization of alginate gel for the entrapment of living whole cells
    25 schema:pagination 393-400
    26 schema:productId N14cba6e8da4c4620a9d9dbccce118ea4
    27 Nf2056f1034134d5e90816194c26ac8c9
    28 Nfc72efc4b16a4cd6979f2a9884f2948f
    29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034600063
    30 https://doi.org/10.1007/bf01134097
    31 schema:sdDatePublished 2019-04-11T13:27
    32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    33 schema:sdPublisher N9cd5508e7a4e4932b1c365bef28c5a81
    34 schema:url http://link.springer.com/10.1007/BF01134097
    35 sgo:license sg:explorer/license/
    36 sgo:sdDataset articles
    37 rdf:type schema:ScholarlyArticle
    38 N14cba6e8da4c4620a9d9dbccce118ea4 schema:name readcube_id
    39 schema:value a4d290222b9f360915221f9e00f57b41ea55b648f416638425ed78aff49428d3
    40 rdf:type schema:PropertyValue
    41 N18fa99e6e4c944edb6f5cd084c03e6e7 rdf:first sg:person.071444746.01
    42 rdf:rest N37541d80b5ca4670a04803ff8fd32bee
    43 N19e444582d5e4e2ab18d238c15e67490 schema:affiliation https://www.grid.ac/institutes/grid.4514.4
    44 schema:familyName Pendleton
    45 schema:givenName Robert
    46 rdf:type schema:Person
    47 N25e204012a7e4980889bb8cd7fd9cf5f schema:issueNumber 8
    48 rdf:type schema:PublicationIssue
    49 N37541d80b5ca4670a04803ff8fd32bee rdf:first sg:person.01362574601.27
    50 rdf:rest rdf:nil
    51 N9cd5508e7a4e4932b1c365bef28c5a81 schema:name Springer Nature - SN SciGraph project
    52 rdf:type schema:Organization
    53 Na548bda68e8243afb695362dd21104d1 rdf:first sg:person.012720513767.08
    54 rdf:rest Ne75c247d21954d0b866f1ff35aca2137
    55 Ne75c247d21954d0b866f1ff35aca2137 rdf:first N19e444582d5e4e2ab18d238c15e67490
    56 rdf:rest N18fa99e6e4c944edb6f5cd084c03e6e7
    57 Nf2056f1034134d5e90816194c26ac8c9 schema:name dimensions_id
    58 schema:value pub.1034600063
    59 rdf:type schema:PropertyValue
    60 Nf4e506f906704f518d2190d17a07767f schema:volumeNumber 3
    61 rdf:type schema:PublicationVolume
    62 Nfc72efc4b16a4cd6979f2a9884f2948f schema:name doi
    63 schema:value 10.1007/bf01134097
    64 rdf:type schema:PropertyValue
    65 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    66 schema:name Biological Sciences
    67 rdf:type schema:DefinedTerm
    68 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    69 schema:name Biochemistry and Cell Biology
    70 rdf:type schema:DefinedTerm
    71 sg:journal.1289037 schema:issn 0951-208X
    72 1573-6784
    73 schema:name Biotechnology Letters
    74 rdf:type schema:Periodical
    75 sg:person.012720513767.08 schema:affiliation https://www.grid.ac/institutes/grid.4514.4
    76 schema:familyName Birnbaum
    77 schema:givenName Staffan
    78 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012720513767.08
    79 rdf:type schema:Person
    80 sg:person.01362574601.27 schema:affiliation https://www.grid.ac/institutes/grid.4514.4
    81 schema:familyName Mosbach
    82 schema:givenName Klaus
    83 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362574601.27
    84 rdf:type schema:Person
    85 sg:person.071444746.01 schema:affiliation https://www.grid.ac/institutes/grid.4514.4
    86 schema:familyName Larsson
    87 schema:givenName Per-Olof
    88 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.071444746.01
    89 rdf:type schema:Person
    90 sg:pub.10.1007/bf00498724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053723453
    91 https://doi.org/10.1007/bf00498724
    92 rdf:type schema:CreativeWork
    93 sg:pub.10.1007/bf00505015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015922032
    94 https://doi.org/10.1007/bf00505015
    95 rdf:type schema:CreativeWork
    96 sg:pub.10.1007/bf01387977 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012803158
    97 https://doi.org/10.1007/bf01387977
    98 rdf:type schema:CreativeWork
    99 sg:pub.10.1007/bf02996744 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044893386
    100 https://doi.org/10.1007/bf02996744
    101 rdf:type schema:CreativeWork
    102 https://doi.org/10.1002/bit.260190309 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047950144
    103 rdf:type schema:CreativeWork
    104 https://doi.org/10.1002/bit.260211202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040101312
    105 rdf:type schema:CreativeWork
    106 https://doi.org/10.1016/0014-5793(80)80463-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034460040
    107 rdf:type schema:CreativeWork
    108 https://doi.org/10.1016/0014-5793(80)81238-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010371868
    109 rdf:type schema:CreativeWork
    110 https://doi.org/10.1016/0141-0229(80)90097-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051737539
    111 rdf:type schema:CreativeWork
    112 https://doi.org/10.1093/oxfordjournals.jbchem.a127107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083613495
    113 rdf:type schema:CreativeWork
    114 https://doi.org/10.1126/science.6776628 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062640201
    115 rdf:type schema:CreativeWork
    116 https://www.grid.ac/institutes/grid.4514.4 schema:alternateName Lund University
    117 schema:name Pure and Applied Biochemistry Chemical Center, University of Lund, P.O. Box 740, S-220 07, Lund, Sweden
    118 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...