Covalent stabilization of alginate gel for the entrapment of living whole cells View Full Text


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Article Info

DATE

1981-08

AUTHORS

Staffan Birnbaum, Robert Pendleton, Per-Olof Larsson, Klaus Mosbach

ABSTRACT

Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients. The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized. More... »

PAGES

393-400

References to SciGraph publications

  • 1977-09. Screening of matrix suitable for immobilization of microbial cells in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1979-06. Steroid transformation by living cells immobilized in calcium alginate in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1980-12. Denitrification of water using immobilized Pseudomonas denitrificans cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1979-12. Alcohol production by magnetic immobilized yeast in BIOTECHNOLOGY LETTERS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf01134097

    DOI

    http://dx.doi.org/10.1007/bf01134097

    DIMENSIONS

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