A fungal chitinase gene fromRhizopus oligosporus confers antifungal activity to transgenic tobacco View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-04

AUTHORS

T. Terakawa, N. Takaya, H. Horiuchi, M. Koike, M. Takagi

ABSTRACT

We have studied whether a chitinase involved in cell autolysis of a filamentous fungus,Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. Thechi1 gene was introduced into tobacco by theAgrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which thechi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogensSclerotinia sclerotiorum andBotrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves. More... »

PAGES

439-443

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01092762

DOI

http://dx.doi.org/10.1007/bf01092762

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010799304

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30727628


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