Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillusmegaterium View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-12

AUTHORS

Kwang H. Son, Jong H. Jang, Jung H. Kim

ABSTRACT

The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture. More... »

PAGES

821-824

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01026189

DOI

http://dx.doi.org/10.1007/bf01026189

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040805698

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28247147


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