Structural determinants of the intrinsic fluorescence emission in notexin and phospholipase A2 enzymes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

Long-sen Chang, Chen-chung Yang

ABSTRACT

Fluorescence measurements of the homologous proteins, notexin and PLA2 enzymes fromNaja naja atra, Naja nigricollis, and Hemachatus haemachatus venoms, showed that the wavelength of maximum emission and the quantum yield of their intrinsic fluorescence emission spectra were different. To verify the factors which affected their fluorescence characteristics, the dynamics of tryptophan residues in those homologous proteins were studied by quenching with acrylamide, iodide, and cesium. The degrees of exposure of tryptophanyl groups in notexin and PLA2 enzymes assessed by acrylamide quenching were found to be the major factor that determined their fluorescence characteristics. However, the positively charged groups surrounding tryptophan residues of PLA2 enzymes fromN. naja atra andN. nigricollis venoms might affect the quantum yield of their fluorophores. Tryptophan residues of notexin were in an environment with less fluctuation, which did not allow free diffusion of ionic quencher. This might render its typtophan residues to fluoresce at a shorter wavelength. These results suggested that the structural determinants affecting the intrinsic fluorescence emission of homologous proteins can be easily assessed by quenching studies. More... »

PAGES

579-583

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01025122

DOI

http://dx.doi.org/10.1007/bf01025122

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032549247

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8142000


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