Effect of calcium chloride treatment on hybridoma cell viability and growth View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-10

AUTHORS

Gyun Min Lee, Bong Kwan Han, Jung Hoe Kim, Bernhard O. Palsson

ABSTRACT

In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and treatment time. The cell viability as measured by trypan blue exclusion did not decrease rapidly during the first hour of calcium chloride treatment regardless of calcium chloride concentrations used (1.3 and 1.5%). However, 1.3% calcium chloride solution appeared to be more detrimental to the cells than 1.5% calcium chloride solution. The cells in 1.3% calcium chloride solution lost their viability faster than the cells in 1.5% calcium chloride solution. In addition, when the cells treated with calcium chloride were inoculated into spinner flasks containing IMDM with 10% fetal calf serum, the cells treated with 1.3% calcium chloride solution showed a longer lag phase than the cells treated with 1.5% calcium chloride solution. More... »

PAGES

891-896

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01020624

DOI

http://dx.doi.org/10.1007/bf01020624

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022045873


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