Cytofluorometric demonstration of a catecholamine in rat mast cells after the administration of DOPA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-03

AUTHORS

Lennart Enerbäck

ABSTRACT

A catecholamine, probably dopamine, was identified in rat peritoneal mast cells after subcutaneous injection of DOPA. Its identity was established by cytofluorometry of cells treated with hot formaldehyde vapour according to the Falck-Hillarp technique. Injections of 50–200 mg/kgl-DOPA were followed by a dose-dependent increase in fluorescence intensity, measured at the emission maximum for catecholamines. The increase in fluorescence intensity was accompanied by a change in the emission spectrum with displacement of the fluorescence maximum towards a shorter wavelength characteristic for a catecholamine. Recordings of rates of photodecomposition showed a rapid exponential fading of the fluorescence in mast cells of control rats comparable to that of 5-hydroxytryptamine-containing protein droplets, whereas the mast cells of DOPA-treated rats showed a slower fading rate, intermediate between that of dopamine- and 5-hydroxytryptamine-containing models. More... »

PAGES

131-142

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf01012555

DOI

http://dx.doi.org/10.1007/bf01012555

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029962366

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/4710030


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