A quantitative assay to measure homotypic vacuole fusion in vitro View Full Text


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Article Info

DATE

1995-12

AUTHORS

Albert Haas

ABSTRACT

Homotypic (self) vacuole fusion is the last discernible step in vacuole inheritance ofSaccharomyces cerevisiae and was recently successfully reconstituted in vitro using purified vacuoles, cytosol, ATP, moderate salt concentrations, and a physiological temperature. The following protocol describes a rapid, reliable, and easy technique to quantitate homotypic vacuole fusion in vitro. Frequency of fusion is determined by quantifying alkaline phosphatase activity after fusion has taken place. This method has been successfully used to analyze pharmacological reagents which interfere with certain subreactions and to identify distinct proteins which play key roles in the fusion events. More... »

PAGES

283-294

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00986234

DOI

http://dx.doi.org/10.1007/bf00986234

DIMENSIONS

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