Arachidonic and docosahexanoic acid content of bovine brain myelin: Implications for the pathogenesis of multiple sclerosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-01

AUTHORS

Khurshed A. Ansari, Don W. Shoeman

ABSTRACT

Lipids were extracted from bovine brain myelin using a mixture of hexane and isopropanol (3:2). Myelin lipids were resolved, using Sep Pak chromatography, into four fractions: Fraction 1 contained neutral lipids, fraction 2, free fatty acids, fraction 3, ethanolamine phospholipids and fraction 4, choline phospholipids. Doscosahexanoic (DHA) and arachidonic (AA) acids in these fractions were measured by RPHPLC. Fraction 2 was analyzed directly, the other three fractions were subjected to alkaline hydrolysis before analysis for DHA and AA. DHA and AA were not found in fraction 1. Both DHA and AA were found in fractions 2 and 3. Only AA was consistently found in fraction 4. These results were confirmed by GC. More... »

PAGES

7-11

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00969177

DOI

http://dx.doi.org/10.1007/bf00969177

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019947998

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2109266


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