Monoclonal antibodies as neural cell surface markers View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-03

AUTHORS

O. K. Langley, M. S. Ghandour, G. Gombos, M. Hirn, C. Goridis

ABSTRACT

A comparison is made of the immunohistochemistry at the ultrastructural level of three monoclonal antibodies directed against surface components of CNS cells. Hybridomas secreting these antibodies were obtained from two cell fusions of a rat myeloma cell line and immune splenocytes derived from rats immunized either with primary mouse brain cultured cells or membrane components. In cultures one antibody, anti-BSP-2 (Brain Surface Protein-2), was preferentially directed against neurones while another, anti-BSP-3 (Brain Surface Protein-3), preferentially labeled astrocytes. In mouse cerebellar sections, both labeled the surface of Purkinje cells, granule cells and astrocytes. In addition a cytoplasm localization was apparent in granule cells and astrocytes. Another antibody anti-MESA-1 (Mouse Endothelial Surface Antigen-1) reacted exclusively with the surface of endothelial cells lining blood vessels. These data are discussed with reference to the biochemical nature of the corresponding antigens and to known glycoproteins of neural cell membranes. More... »

PAGES

349-362

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00965646

DOI

http://dx.doi.org/10.1007/bf00965646

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010597676

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7050755


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