Double ligand ELISA technique for the estimation of antibodies to brain tissue antigens in patients with neurological disorders View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-04

AUTHORS

Liane Bologa, Willy Hofstetter, Katharina Schonenberger, Claude Jacque, Norbert Herschkowitz

ABSTRACT

A double ligand enzyme-linked immunosorbent assay (ELISA) has been developed to detect antibodies against brain tissue antigens in the sera of patients with neurological diseases. The sera were tested on human white matter homogenate. The technique consists of successive incubations with the human serum to be tested, rabbit immunoglobulin G (IgG) to human immunoglobulins (Ig), alkaline phosphate-labeled protein A and alkaline phosphatase substrate. This procedure has the advantage of increased sensitivity compared to the classical ELISA. Application of this procedure to the sera of patients with neurological diseases showed that the unspecific binding is very low and the results are reliable. Moreover the test allows the detection of antibodies to chemically different antigenic structures that can occur in a variety of neurological diseases. More... »

PAGES

469-481

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00964651

DOI

http://dx.doi.org/10.1007/bf00964651

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1042989420

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3889690


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