Fatty acid chain is a critical epitope for antiphospholipid antibody View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-05

AUTHORS

Roger A. Levy, Azzudin E. Gharavi, Lisa R. Sammaritano, Ladislav Habina, Michael D. Lockshin

ABSTRACT

To explore the role of phospholipid fatty acids in binding of antiphospholipid antibody (aPL) in ELISA, we tested aPL binding to phospholipids containing fatty acids of varying chain length and degree of saturation using direct ELISA and inhibition methods. Polyclonal IgG and IgM human aPL's bind to C18:1 phosphatidylglycerol (PG) better than to C18:0 PG or C18:2 PG. Binding is greater to C18 than to C14:0 or C16:0 PGs; aPL's do not bind to C12:0 PG. aPL binding is not inhibited by C18:1 diacylglycerol, glycerol-3-phosphate, myoinositol, or myoinositol phosphate. The fatty acid chains are critical determinants for antigen recognition and, by projection, biological activity of aPL. More... »

PAGES

141-145

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00917913

DOI

http://dx.doi.org/10.1007/bf00917913

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040024079

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1694860


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