Evaluation of image analysis and laser granulometry for microbial cell sizing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-06

AUTHORS

J. Vaija, A. Lagaude, C. Ghommidh

ABSTRACT

A direct cell size measurement technique and an image analysis based sizing method were developed. The former consisted of a manual size measurement of the two-dimensional cell images on a video screen, with automatic data recording. This method was chosen as the reference. The latter, a semiautomatic method took advantage of a commercial computer program designed for image processing and particle morphology analysis. It gave average and median size values which were compatible with the manual method. However, the performance of these time consuming methods is limited. Hence, the laser granulometry technique, intrinsically far more powerful while capable of analysing millions of sample objects in a short time delay, was applied. The comparison revealed that this method gives too low size values, particularly in disagreement with the known dimensions of the bacterial (Zymomonas mobilis) cells. A size correction method was developed to realign the granulometry results of Z mobilis cell samples with those of the direct manual measurement method. More... »

PAGES

139-149

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00871209

DOI

http://dx.doi.org/10.1007/bf00871209

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027132470

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7771762


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