Interspecific somatic hybrids ofRudbeckia hirta andR. Laciniata (Compositae) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-01

AUTHORS

J. S. Al-Atabee, B. J. Mulligan, J. B. Power

ABSTRACT

Interspecific somatic hybrid plants betweenRudbeckia hirta cv. Marmalade andR.laciniata cv. Irish Eyes were regenerated following the electro-fusion of mesophyll protoplasts ofR.hirta with callus protoplasts ofR.laciniata. A hybrid selection scheme was based on the fact that plant regeneration, from parental protoplasts ofR.hirta, was via shoot regeneration of callus, and only via rhizogenesis forR.laciniata. The other half of the selection strategy was based on the presence of anthocyanin-pigmented roots; a characteristic of theR.hirta parent only. Somatic hybrids were regenerated, via rhizogenesis, alongside normalR.laciniata but were distinguished by the presence of pigmented roots (a feature ofR.hirta). Hybrid plants had a floral morphology that was intermediate as compared to that of the two parents, with an expected somatic chromosome number of 2n=(2x+4x)=74. Pollen viability though was low. Esterase and peroxidase isozyme profiles confirmed the hybrid nature of the regenerated plants with pigmented roots, whilst chloroplast DNA restriction analysis showed that these hybrids had aR.laciniata chloroplast DNA. This demonstration of somatic hybridisation not only opens up the possibility of incorporating novel traits between such ornamentalCompositae species, but provides a selection strategy based on rhizogenesis as the route to plant regeneration coupled with heritable pigmentation production of roots as a confirmatory hybrid marker. More... »

PAGES

517-520

Journal

TITLE

Plant Cell Reports

ISSUE

9

VOLUME

8

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00820199

DOI

http://dx.doi.org/10.1007/bf00820199

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030208162

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24226276


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