Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-07

AUTHORS

R. Peerbolte, K. Leenhouts, G. M. S. Hooykaas-van Slogteren, G. J. Wullems, R. A. Schilperoort

ABSTRACT

Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an expected phenotype (phytohormone independent growth in tissue culture (Aut+), shoot regeneration (Reg+) and octopine synthesis (Ocs+)), clones were obtained with an aberrant phenotype. One of these clones, TSO38, is Aut+Reg+ but shows little or no octopine synthesis activity (Ocs-). Subclones of TSO38, however, are either Ocs- or Ocs+. Ocs- shoots become Ocs+ under certain states of differentiation, indicating that the octopine synthase gene is present. The fact that in the Ocs- subclones the octopine synthase gene is not expressed, is probably due to DNA methylation (29). The present paper describes that shoots derived from both an Ocs+ and an Ocs- subclone of TSO38, which were negative for the presence of mannopine (Mas-) and agropine (Ags-), became Mas+Ags+ after culturing on medium containing the hypomethylating agent 5-azacytidine. This means that both in the Ocs- line and in the Ocs+ line expression of TR-DNA opine genes most likely was hampered by DNA methylation. The T-DNA structures of an Ocs- and an Ocs+ TSO38 subclone proved to be identical and surprisingly complex. No intact copy of Tn1831 was present. TL-DNA and TR-DNA segments, present in high copy numbers, were truncated; several T-DNA segments existed in tandem arrangements. When DNA from an Ocs+ and an Ocs- subclone of TSO38 were compared for cleavability by the methylation sensitive restriction enzymes HpaII and MspII, differences were detected, but it became also clear that both lines contained methylated T-DNA segments. This indicates that the Ocs- and the Ocs+ TSO38 subclones differ only quantitatively in respect to degree of T-DNA methylation. More... »

PAGES

285-299

References to SciGraph publications

  • 1983-06. Nopaline T-DNA is maintained during regeneration and generative propagation of transformed tobacco plants in MOLECULAR GENETICS AND GENOMICS
  • 1984-11. Silent T-DNA genes in plant lines transformed by Agrobacterium tumefaciens are activated by grafting and by 5-azacytidine treatment in PLANT MOLECULAR BIOLOGY
  • 1981-08. Sequence specificity of methylation in higher plant DNA in NATURE
  • 1982-02. Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells in NATURE
  • 1985-07. Transformation of plant protoplasts with DNA: cotransformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences in PLANT MOLECULAR BIOLOGY
  • 1986-07. Clones from a shooty tobacco crown gall tumor I: deletions, rearrangements and amplifications resulting in irregular T-DNA structures and organizations in PLANT MOLECULAR BIOLOGY
  • 1973-07. Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco in NATURE
  • 1983-11. Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955 in PLANT MOLECULAR BIOLOGY
  • 1983-11. Clonal analysis of heterogeneous crown gall tumor tissues induced by wild-type and shooter mutant strains ofAgrobacterium tumefaciens-expression of T-DNA genes in PLANT MOLECULAR BIOLOGY
  • 1984-11. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line in MOLECULAR GENETICS AND GENOMICS
  • 1984-06. Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae in MOLECULAR GENETICS AND GENOMICS
  • 1980-09. The expression of tumour markers in intraspecific somatic hybrids of normal and crown gall cells from Nicotiana tabacum in THEORETICAL AND APPLIED GENETICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00752901

    DOI

    http://dx.doi.org/10.1007/bf00752901

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1025672137

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24302370


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands", 
              "id": "http://www.grid.ac/institutes/grid.5132.5", 
              "name": [
                "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Peerbolte", 
            "givenName": "R.", 
            "id": "sg:person.01245044405.98", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245044405.98"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands", 
              "id": "http://www.grid.ac/institutes/grid.5132.5", 
              "name": [
                "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Leenhouts", 
            "givenName": "K.", 
            "id": "sg:person.01241000760.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241000760.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands", 
              "id": "http://www.grid.ac/institutes/grid.5132.5", 
              "name": [
                "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hooykaas-van Slogteren", 
            "givenName": "G. M. S.", 
            "id": "sg:person.01307114160.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307114160.14"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands", 
              "id": "http://www.grid.ac/institutes/grid.5132.5", 
              "name": [
                "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Wullems", 
            "givenName": "G. J.", 
            "id": "sg:person.01174342640.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174342640.26"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands", 
              "id": "http://www.grid.ac/institutes/grid.5132.5", 
              "name": [
                "Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schilperoort", 
            "givenName": "R. A.", 
            "id": "sg:person.014503026714.34", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014503026714.34"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/295432a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033213612", 
              "https://doi.org/10.1038/295432a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/newbio244029a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022855491", 
              "https://doi.org/10.1038/newbio244029a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01578594", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040431841", 
              "https://doi.org/10.1007/bf01578594"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00752900", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028602163", 
              "https://doi.org/10.1007/bf00752900"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00331085", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044783076", 
              "https://doi.org/10.1007/bf00331085"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00332748", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030665370", 
              "https://doi.org/10.1007/bf00332748"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00295450", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027399182", 
              "https://doi.org/10.1007/bf00295450"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00329940", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037063138", 
              "https://doi.org/10.1007/bf00329940"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00020641", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026807225", 
              "https://doi.org/10.1007/bf00020641"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00033379", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028552579", 
              "https://doi.org/10.1007/bf00033379"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf01578595", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046059264", 
              "https://doi.org/10.1007/bf01578595"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/292860a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024461091", 
              "https://doi.org/10.1038/292860a0"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1986-07", 
        "datePublishedReg": "1986-07-01", 
        "description": "Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an expected phenotype (phytohormone independent growth in tissue culture (Aut+), shoot regeneration (Reg+) and octopine synthesis (Ocs+)), clones were obtained with an aberrant phenotype. One of these clones, TSO38, is Aut+Reg+ but shows little or no octopine synthesis activity (Ocs-). Subclones of TSO38, however, are either Ocs- or Ocs+. Ocs- shoots become Ocs+ under certain states of differentiation, indicating that the octopine synthase gene is present. The fact that in the Ocs- subclones the octopine synthase gene is not expressed, is probably due to DNA methylation (29). The present paper describes that shoots derived from both an Ocs+ and an Ocs- subclone of TSO38, which were negative for the presence of mannopine (Mas-) and agropine (Ags-), became Mas+Ags+ after culturing on medium containing the hypomethylating agent 5-azacytidine. This means that both in the Ocs- line and in the Ocs+ line expression of TR-DNA opine genes most likely was hampered by DNA methylation. The T-DNA structures of an Ocs- and an Ocs+ TSO38 subclone proved to be identical and surprisingly complex. No intact copy of Tn1831 was present. TL-DNA and TR-DNA segments, present in high copy numbers, were truncated; several T-DNA segments existed in tandem arrangements. When DNA from an Ocs+ and an Ocs- subclone of TSO38 were compared for cleavability by the methylation sensitive restriction enzymes HpaII and MspII, differences were detected, but it became also clear that both lines contained methylated T-DNA segments. This indicates that the Ocs- and the Ocs+ TSO38 subclones differ only quantitatively in respect to degree of T-DNA methylation.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/bf00752901", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1101246", 
            "issn": [
              "0167-4412", 
              "1573-5028"
            ], 
            "name": "Plant Molecular Biology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "keywords": [
          "T-DNA structure", 
          "octopine synthase gene", 
          "T-DNA segments", 
          "synthase gene", 
          "DNA methylation", 
          "methylation-sensitive restriction", 
          "crown gall tumors", 
          "tobacco crown gall tumor", 
          "high copy number", 
          "TL-DNA", 
          "intact copy", 
          "gall tumors", 
          "byAgrobacterium tumefaciens", 
          "conditional expression", 
          "aberrant phenotypes", 
          "copy number", 
          "genes", 
          "methylation", 
          "clones", 
          "TL region", 
          "line expression", 
          "subclones", 
          "synthesis activity", 
          "expression", 
          "phenotype", 
          "tandem arrangement", 
          "OC lines", 
          "tumefaciens", 
          "shoots", 
          "loci", 
          "HpaII", 
          "DNA", 
          "differentiation", 
          "copies", 
          "cleavability", 
          "segments", 
          "structure", 
          "insertion", 
          "lines", 
          "activity", 
          "presence", 
          "addition", 
          "medium", 
          "number", 
          "agents", 
          "organization", 
          "arrangement", 
          "tumors", 
          "differences", 
          "OC", 
          "fact", 
          "degree", 
          "restriction", 
          "respect", 
          "state", 
          "present paper", 
          "certain states", 
          "paper"
        ], 
        "name": "Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes", 
        "pagination": "285-299", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1025672137"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00752901"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24302370"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00752901", 
          "https://app.dimensions.ai/details/publication/pub.1025672137"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-09-02T15:47", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_213.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/bf00752901"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00752901'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00752901'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00752901'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00752901'


     

    This table displays all metadata directly associated to this object as RDF triples.

    195 TRIPLES      21 PREDICATES      96 URIs      76 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00752901 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Nb7871a9b7dd744fbb429e516481c5b3a
    4 schema:citation sg:pub.10.1007/bf00020641
    5 sg:pub.10.1007/bf00033379
    6 sg:pub.10.1007/bf00295450
    7 sg:pub.10.1007/bf00329940
    8 sg:pub.10.1007/bf00331085
    9 sg:pub.10.1007/bf00332748
    10 sg:pub.10.1007/bf00752900
    11 sg:pub.10.1007/bf01578594
    12 sg:pub.10.1007/bf01578595
    13 sg:pub.10.1038/292860a0
    14 sg:pub.10.1038/295432a0
    15 sg:pub.10.1038/newbio244029a0
    16 schema:datePublished 1986-07
    17 schema:datePublishedReg 1986-07-01
    18 schema:description Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an expected phenotype (phytohormone independent growth in tissue culture (Aut+), shoot regeneration (Reg+) and octopine synthesis (Ocs+)), clones were obtained with an aberrant phenotype. One of these clones, TSO38, is Aut+Reg+ but shows little or no octopine synthesis activity (Ocs-). Subclones of TSO38, however, are either Ocs- or Ocs+. Ocs- shoots become Ocs+ under certain states of differentiation, indicating that the octopine synthase gene is present. The fact that in the Ocs- subclones the octopine synthase gene is not expressed, is probably due to DNA methylation (29). The present paper describes that shoots derived from both an Ocs+ and an Ocs- subclone of TSO38, which were negative for the presence of mannopine (Mas-) and agropine (Ags-), became Mas+Ags+ after culturing on medium containing the hypomethylating agent 5-azacytidine. This means that both in the Ocs- line and in the Ocs+ line expression of TR-DNA opine genes most likely was hampered by DNA methylation. The T-DNA structures of an Ocs- and an Ocs+ TSO38 subclone proved to be identical and surprisingly complex. No intact copy of Tn1831 was present. TL-DNA and TR-DNA segments, present in high copy numbers, were truncated; several T-DNA segments existed in tandem arrangements. When DNA from an Ocs+ and an Ocs- subclone of TSO38 were compared for cleavability by the methylation sensitive restriction enzymes HpaII and MspII, differences were detected, but it became also clear that both lines contained methylated T-DNA segments. This indicates that the Ocs- and the Ocs+ TSO38 subclones differ only quantitatively in respect to degree of T-DNA methylation.
    19 schema:genre article
    20 schema:isAccessibleForFree false
    21 schema:isPartOf N3fd40c2410384c1c8de8cc69eece968a
    22 Nb8f570fe7e1b474292ef5c76aa168a4e
    23 sg:journal.1101246
    24 schema:keywords DNA
    25 DNA methylation
    26 HpaII
    27 OC
    28 OC lines
    29 T-DNA segments
    30 T-DNA structure
    31 TL region
    32 TL-DNA
    33 aberrant phenotypes
    34 activity
    35 addition
    36 agents
    37 arrangement
    38 byAgrobacterium tumefaciens
    39 certain states
    40 cleavability
    41 clones
    42 conditional expression
    43 copies
    44 copy number
    45 crown gall tumors
    46 degree
    47 differences
    48 differentiation
    49 expression
    50 fact
    51 gall tumors
    52 genes
    53 high copy number
    54 insertion
    55 intact copy
    56 line expression
    57 lines
    58 loci
    59 medium
    60 methylation
    61 methylation-sensitive restriction
    62 number
    63 octopine synthase gene
    64 organization
    65 paper
    66 phenotype
    67 presence
    68 present paper
    69 respect
    70 restriction
    71 segments
    72 shoots
    73 state
    74 structure
    75 subclones
    76 synthase gene
    77 synthesis activity
    78 tandem arrangement
    79 tobacco crown gall tumor
    80 tumefaciens
    81 tumors
    82 schema:name Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes
    83 schema:pagination 285-299
    84 schema:productId N29ece3a52b3a4d30a62d8c875bb7b945
    85 N60a7643907094e9194cf4ec8e783e314
    86 N62a473d9d30c4d14a8490deee788d754
    87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025672137
    88 https://doi.org/10.1007/bf00752901
    89 schema:sdDatePublished 2022-09-02T15:47
    90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    91 schema:sdPublisher N3420433f15394eec831cb3cc4e5b70b3
    92 schema:url https://doi.org/10.1007/bf00752901
    93 sgo:license sg:explorer/license/
    94 sgo:sdDataset articles
    95 rdf:type schema:ScholarlyArticle
    96 N29ece3a52b3a4d30a62d8c875bb7b945 schema:name dimensions_id
    97 schema:value pub.1025672137
    98 rdf:type schema:PropertyValue
    99 N3420433f15394eec831cb3cc4e5b70b3 schema:name Springer Nature - SN SciGraph project
    100 rdf:type schema:Organization
    101 N3fd40c2410384c1c8de8cc69eece968a schema:volumeNumber 7
    102 rdf:type schema:PublicationVolume
    103 N5176fc783dba4556bcc6e0d5a0cb3732 rdf:first sg:person.014503026714.34
    104 rdf:rest rdf:nil
    105 N60a7643907094e9194cf4ec8e783e314 schema:name pubmed_id
    106 schema:value 24302370
    107 rdf:type schema:PropertyValue
    108 N62a473d9d30c4d14a8490deee788d754 schema:name doi
    109 schema:value 10.1007/bf00752901
    110 rdf:type schema:PropertyValue
    111 N7d2e1f43140d4b168331f0f500207412 rdf:first sg:person.01174342640.26
    112 rdf:rest N5176fc783dba4556bcc6e0d5a0cb3732
    113 Nb1fdefafcad7465094603c9e26d8db46 rdf:first sg:person.01307114160.14
    114 rdf:rest N7d2e1f43140d4b168331f0f500207412
    115 Nb7871a9b7dd744fbb429e516481c5b3a rdf:first sg:person.01245044405.98
    116 rdf:rest Ne56ac50204f44de48ae087fccbfefd26
    117 Nb8f570fe7e1b474292ef5c76aa168a4e schema:issueNumber 4
    118 rdf:type schema:PublicationIssue
    119 Ne56ac50204f44de48ae087fccbfefd26 rdf:first sg:person.01241000760.64
    120 rdf:rest Nb1fdefafcad7465094603c9e26d8db46
    121 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Biological Sciences
    123 rdf:type schema:DefinedTerm
    124 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Genetics
    126 rdf:type schema:DefinedTerm
    127 sg:journal.1101246 schema:issn 0167-4412
    128 1573-5028
    129 schema:name Plant Molecular Biology
    130 schema:publisher Springer Nature
    131 rdf:type schema:Periodical
    132 sg:person.01174342640.26 schema:affiliation grid-institutes:grid.5132.5
    133 schema:familyName Wullems
    134 schema:givenName G. J.
    135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174342640.26
    136 rdf:type schema:Person
    137 sg:person.01241000760.64 schema:affiliation grid-institutes:grid.5132.5
    138 schema:familyName Leenhouts
    139 schema:givenName K.
    140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241000760.64
    141 rdf:type schema:Person
    142 sg:person.01245044405.98 schema:affiliation grid-institutes:grid.5132.5
    143 schema:familyName Peerbolte
    144 schema:givenName R.
    145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245044405.98
    146 rdf:type schema:Person
    147 sg:person.01307114160.14 schema:affiliation grid-institutes:grid.5132.5
    148 schema:familyName Hooykaas-van Slogteren
    149 schema:givenName G. M. S.
    150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01307114160.14
    151 rdf:type schema:Person
    152 sg:person.014503026714.34 schema:affiliation grid-institutes:grid.5132.5
    153 schema:familyName Schilperoort
    154 schema:givenName R. A.
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014503026714.34
    156 rdf:type schema:Person
    157 sg:pub.10.1007/bf00020641 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026807225
    158 https://doi.org/10.1007/bf00020641
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1007/bf00033379 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028552579
    161 https://doi.org/10.1007/bf00033379
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1007/bf00295450 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027399182
    164 https://doi.org/10.1007/bf00295450
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1007/bf00329940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037063138
    167 https://doi.org/10.1007/bf00329940
    168 rdf:type schema:CreativeWork
    169 sg:pub.10.1007/bf00331085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044783076
    170 https://doi.org/10.1007/bf00331085
    171 rdf:type schema:CreativeWork
    172 sg:pub.10.1007/bf00332748 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030665370
    173 https://doi.org/10.1007/bf00332748
    174 rdf:type schema:CreativeWork
    175 sg:pub.10.1007/bf00752900 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028602163
    176 https://doi.org/10.1007/bf00752900
    177 rdf:type schema:CreativeWork
    178 sg:pub.10.1007/bf01578594 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040431841
    179 https://doi.org/10.1007/bf01578594
    180 rdf:type schema:CreativeWork
    181 sg:pub.10.1007/bf01578595 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046059264
    182 https://doi.org/10.1007/bf01578595
    183 rdf:type schema:CreativeWork
    184 sg:pub.10.1038/292860a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024461091
    185 https://doi.org/10.1038/292860a0
    186 rdf:type schema:CreativeWork
    187 sg:pub.10.1038/295432a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033213612
    188 https://doi.org/10.1038/295432a0
    189 rdf:type schema:CreativeWork
    190 sg:pub.10.1038/newbio244029a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022855491
    191 https://doi.org/10.1038/newbio244029a0
    192 rdf:type schema:CreativeWork
    193 grid-institutes:grid.5132.5 schema:alternateName Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    194 schema:name Dept. of Plant Molecular Biology, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
    195 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...