Messenger RNA encoding a glutathione-S-transferase responsible for herbicide tolerance in maize is induced in response to safener treatment View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-07

AUTHORS

Roger C. Wiegand, Dilip M. Shah, Thomas J. Mozer, Elizabeth I. Harding, Judith Diaz-Collier, Court Saunders, Ernest G. Jaworski, David C. Tiemeier

ABSTRACT

Glutathione-S-transferases (GST's) in maize represent a family of enzymes which conjugate glutathione to several major classes of pre-emergent, selective herbicides. Chemicals termed safeners have been demonstrated to increase the tolerance of maize toward such herbicides when the maize seed has been previously treated with safeners. It has subsequently been shown that corresponding increases in glutathione-S-transferase species occur. To determine whether these compounds act at a transcriptional level we have used synthetic oligonucleotide probes to isolate cDNA clones encoding the major GST polypeptide subunit, designated GST A. The identity of the clones has been confirmed by hybrid-selected mRNA translation and immunoprecipitation using antibodies made against this GST species as well as by production of active GST in yeast cells transformed with an expression vector containing the cloned DNA. GST A has been found to be encoded in a mRNA of 1.1 kb. Sequencing of cDNA products obtained by primer extension of maize mRNA using our oligonucleotide probes is consistent with this mRNA corresponding to the isolated cDNA clone. Using the clone as a probe for Northern analysis we have found a three to four-fold increase in the steady state level of this mRNA in maize tissue grown from safener-treated seeds. The level of safener which gives this induction is comparable to that required to obtain herbicide tolerance in the field. More... »

PAGES

235-243

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00752897

DOI

http://dx.doi.org/10.1007/bf00752897

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030676381

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24302366


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wiegand", 
        "givenName": "Roger C.", 
        "id": "sg:person.0623467414.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623467414.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shah", 
        "givenName": "Dilip M.", 
        "id": "sg:person.01007562730.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007562730.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mozer", 
        "givenName": "Thomas J.", 
        "id": "sg:person.01311562333.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311562333.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harding", 
        "givenName": "Elizabeth I.", 
        "id": "sg:person.01052576060.90", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052576060.90"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Diaz-Collier", 
        "givenName": "Judith", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saunders", 
        "givenName": "Court", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jaworski", 
        "givenName": "Ernest G.", 
        "id": "sg:person.0634271433.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634271433.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA", 
          "id": "http://www.grid.ac/institutes/grid.418554.9", 
          "name": [
            "Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tiemeier", 
        "givenName": "David C.", 
        "id": "sg:person.01357675533.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357675533.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00015226", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010526736", 
          "https://doi.org/10.1007/bf00015226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00388845", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013875377", 
          "https://doi.org/10.1007/bf00388845"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1986-07", 
    "datePublishedReg": "1986-07-01", 
    "description": "Glutathione-S-transferases (GST's) in maize represent a family of enzymes which conjugate glutathione to several major classes of pre-emergent, selective herbicides. Chemicals termed safeners have been demonstrated to increase the tolerance of maize toward such herbicides when the maize seed has been previously treated with safeners. It has subsequently been shown that corresponding increases in glutathione-S-transferase species occur. To determine whether these compounds act at a transcriptional level we have used synthetic oligonucleotide probes to isolate cDNA clones encoding the major GST polypeptide subunit, designated GST A. The identity of the clones has been confirmed by hybrid-selected mRNA translation and immunoprecipitation using antibodies made against this GST species as well as by production of active GST in yeast cells transformed with an expression vector containing the cloned DNA. GST A has been found to be encoded in a mRNA of 1.1 kb. Sequencing of cDNA products obtained by primer extension of maize mRNA using our oligonucleotide probes is consistent with this mRNA corresponding to the isolated cDNA clone. Using the clone as a probe for Northern analysis we have found a three to four-fold increase in the steady state level of this mRNA in maize tissue grown from safener-treated seeds. The level of safener which gives this induction is comparable to that required to obtain herbicide tolerance in the field.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00752897", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "keywords": [
      "herbicide tolerance", 
      "cDNA clones", 
      "tolerance of maize", 
      "oligonucleotide probes", 
      "family of enzymes", 
      "maize mRNA", 
      "hybrid-selected mRNA translation", 
      "mRNA translation", 
      "glutathione S-transferase", 
      "steady-state levels", 
      "maize tissues", 
      "synthetic oligonucleotide probes", 
      "safener treatment", 
      "transcriptional level", 
      "active GST", 
      "major GST", 
      "yeast cells", 
      "Northern analysis", 
      "such herbicides", 
      "expression vector", 
      "primer extension", 
      "maize seeds", 
      "selective herbicides", 
      "messenger RNA", 
      "cDNA products", 
      "clones", 
      "safeners", 
      "maize", 
      "mRNA", 
      "major classes", 
      "species", 
      "seeds", 
      "GST", 
      "herbicides", 
      "tolerance", 
      "GST species", 
      "GST A", 
      "immunoprecipitation", 
      "subunits", 
      "kb", 
      "RNA", 
      "sequencing", 
      "DNA", 
      "probe", 
      "enzyme", 
      "four-fold increase", 
      "corresponding increase", 
      "cells", 
      "induction", 
      "family", 
      "glutathione", 
      "state level", 
      "translation", 
      "levels", 
      "tissue", 
      "production", 
      "identity", 
      "A.", 
      "vector", 
      "response", 
      "chemicals", 
      "antibodies", 
      "increase", 
      "compounds", 
      "analysis", 
      "products", 
      "class", 
      "treatment", 
      "extension", 
      "field"
    ], 
    "name": "Messenger RNA encoding a glutathione-S-transferase responsible for herbicide tolerance in maize is induced in response to safener treatment", 
    "pagination": "235-243", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030676381"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00752897"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24302366"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00752897", 
      "https://app.dimensions.ai/details/publication/pub.1030676381"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_217.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00752897"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00752897'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00752897'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00752897'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00752897'


 

This table displays all metadata directly associated to this object as RDF triples.

186 TRIPLES      21 PREDICATES      98 URIs      88 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00752897 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N3f1f0031861a494ea9750553dfb98a6b
4 schema:citation sg:pub.10.1007/bf00015226
5 sg:pub.10.1007/bf00388845
6 schema:datePublished 1986-07
7 schema:datePublishedReg 1986-07-01
8 schema:description Glutathione-S-transferases (GST's) in maize represent a family of enzymes which conjugate glutathione to several major classes of pre-emergent, selective herbicides. Chemicals termed safeners have been demonstrated to increase the tolerance of maize toward such herbicides when the maize seed has been previously treated with safeners. It has subsequently been shown that corresponding increases in glutathione-S-transferase species occur. To determine whether these compounds act at a transcriptional level we have used synthetic oligonucleotide probes to isolate cDNA clones encoding the major GST polypeptide subunit, designated GST A. The identity of the clones has been confirmed by hybrid-selected mRNA translation and immunoprecipitation using antibodies made against this GST species as well as by production of active GST in yeast cells transformed with an expression vector containing the cloned DNA. GST A has been found to be encoded in a mRNA of 1.1 kb. Sequencing of cDNA products obtained by primer extension of maize mRNA using our oligonucleotide probes is consistent with this mRNA corresponding to the isolated cDNA clone. Using the clone as a probe for Northern analysis we have found a three to four-fold increase in the steady state level of this mRNA in maize tissue grown from safener-treated seeds. The level of safener which gives this induction is comparable to that required to obtain herbicide tolerance in the field.
9 schema:genre article
10 schema:isAccessibleForFree false
11 schema:isPartOf N2a63a2dc04bf483a86faeb7ee8c44162
12 Nb238150c8935455d84cb14e13000c3cd
13 sg:journal.1101246
14 schema:keywords A.
15 DNA
16 GST
17 GST A
18 GST species
19 Northern analysis
20 RNA
21 active GST
22 analysis
23 antibodies
24 cDNA clones
25 cDNA products
26 cells
27 chemicals
28 class
29 clones
30 compounds
31 corresponding increase
32 enzyme
33 expression vector
34 extension
35 family
36 family of enzymes
37 field
38 four-fold increase
39 glutathione
40 glutathione S-transferase
41 herbicide tolerance
42 herbicides
43 hybrid-selected mRNA translation
44 identity
45 immunoprecipitation
46 increase
47 induction
48 kb
49 levels
50 mRNA
51 mRNA translation
52 maize
53 maize mRNA
54 maize seeds
55 maize tissues
56 major GST
57 major classes
58 messenger RNA
59 oligonucleotide probes
60 primer extension
61 probe
62 production
63 products
64 response
65 safener treatment
66 safeners
67 seeds
68 selective herbicides
69 sequencing
70 species
71 state level
72 steady-state levels
73 subunits
74 such herbicides
75 synthetic oligonucleotide probes
76 tissue
77 tolerance
78 tolerance of maize
79 transcriptional level
80 translation
81 treatment
82 vector
83 yeast cells
84 schema:name Messenger RNA encoding a glutathione-S-transferase responsible for herbicide tolerance in maize is induced in response to safener treatment
85 schema:pagination 235-243
86 schema:productId Nbb8271888ecf4b928fdaeec622b1c23a
87 Nc397ee03941a4a9bb63715a39ea15a7f
88 Nee2e72da7dbf4b548c99db834256ee93
89 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030676381
90 https://doi.org/10.1007/bf00752897
91 schema:sdDatePublished 2022-08-04T16:51
92 schema:sdLicense https://scigraph.springernature.com/explorer/license/
93 schema:sdPublisher N4b5730c9a7ec430bb95ff126bddec9fb
94 schema:url https://doi.org/10.1007/bf00752897
95 sgo:license sg:explorer/license/
96 sgo:sdDataset articles
97 rdf:type schema:ScholarlyArticle
98 N0daf5696880f4245b4f403df685279d3 rdf:first sg:person.01311562333.02
99 rdf:rest Nf7fbfed8ae22487291b6447e31170099
100 N2a63a2dc04bf483a86faeb7ee8c44162 schema:issueNumber 4
101 rdf:type schema:PublicationIssue
102 N37723900fc39443297022b5e30c72358 schema:affiliation grid-institutes:grid.418554.9
103 schema:familyName Saunders
104 schema:givenName Court
105 rdf:type schema:Person
106 N39376981b8c542e5a6b50d0adf3e4897 rdf:first sg:person.01357675533.28
107 rdf:rest rdf:nil
108 N3f1f0031861a494ea9750553dfb98a6b rdf:first sg:person.0623467414.25
109 rdf:rest Na99f433988cf48d6800a5ceb7b12ca55
110 N4b5730c9a7ec430bb95ff126bddec9fb schema:name Springer Nature - SN SciGraph project
111 rdf:type schema:Organization
112 N578b2a383f824d4c95869f0dcd69e51e rdf:first N9559bd37751e4b67ae9066c6b0e2db83
113 rdf:rest Nce7172cac1a64f3ba93ed1e5c5277562
114 N6d722e264d7d4818948f91340763be6b rdf:first sg:person.0634271433.03
115 rdf:rest N39376981b8c542e5a6b50d0adf3e4897
116 N9559bd37751e4b67ae9066c6b0e2db83 schema:affiliation grid-institutes:grid.418554.9
117 schema:familyName Diaz-Collier
118 schema:givenName Judith
119 rdf:type schema:Person
120 Na99f433988cf48d6800a5ceb7b12ca55 rdf:first sg:person.01007562730.23
121 rdf:rest N0daf5696880f4245b4f403df685279d3
122 Nb238150c8935455d84cb14e13000c3cd schema:volumeNumber 7
123 rdf:type schema:PublicationVolume
124 Nbb8271888ecf4b928fdaeec622b1c23a schema:name doi
125 schema:value 10.1007/bf00752897
126 rdf:type schema:PropertyValue
127 Nc397ee03941a4a9bb63715a39ea15a7f schema:name pubmed_id
128 schema:value 24302366
129 rdf:type schema:PropertyValue
130 Nce7172cac1a64f3ba93ed1e5c5277562 rdf:first N37723900fc39443297022b5e30c72358
131 rdf:rest N6d722e264d7d4818948f91340763be6b
132 Nee2e72da7dbf4b548c99db834256ee93 schema:name dimensions_id
133 schema:value pub.1030676381
134 rdf:type schema:PropertyValue
135 Nf7fbfed8ae22487291b6447e31170099 rdf:first sg:person.01052576060.90
136 rdf:rest N578b2a383f824d4c95869f0dcd69e51e
137 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
138 schema:name Biological Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
141 schema:name Genetics
142 rdf:type schema:DefinedTerm
143 sg:journal.1101246 schema:issn 0167-4412
144 1573-5028
145 schema:name Plant Molecular Biology
146 schema:publisher Springer Nature
147 rdf:type schema:Periodical
148 sg:person.01007562730.23 schema:affiliation grid-institutes:grid.418554.9
149 schema:familyName Shah
150 schema:givenName Dilip M.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01007562730.23
152 rdf:type schema:Person
153 sg:person.01052576060.90 schema:affiliation grid-institutes:grid.418554.9
154 schema:familyName Harding
155 schema:givenName Elizabeth I.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01052576060.90
157 rdf:type schema:Person
158 sg:person.01311562333.02 schema:affiliation grid-institutes:grid.418554.9
159 schema:familyName Mozer
160 schema:givenName Thomas J.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311562333.02
162 rdf:type schema:Person
163 sg:person.01357675533.28 schema:affiliation grid-institutes:grid.418554.9
164 schema:familyName Tiemeier
165 schema:givenName David C.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01357675533.28
167 rdf:type schema:Person
168 sg:person.0623467414.25 schema:affiliation grid-institutes:grid.418554.9
169 schema:familyName Wiegand
170 schema:givenName Roger C.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0623467414.25
172 rdf:type schema:Person
173 sg:person.0634271433.03 schema:affiliation grid-institutes:grid.418554.9
174 schema:familyName Jaworski
175 schema:givenName Ernest G.
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0634271433.03
177 rdf:type schema:Person
178 sg:pub.10.1007/bf00015226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010526736
179 https://doi.org/10.1007/bf00015226
180 rdf:type schema:CreativeWork
181 sg:pub.10.1007/bf00388845 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013875377
182 https://doi.org/10.1007/bf00388845
183 rdf:type schema:CreativeWork
184 grid-institutes:grid.418554.9 schema:alternateName Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA
185 schema:name Biological Sciences Department, Monsanto Company, 700 Chesterfield Village Parkway, 63198, St. Louis, MO, USA
186 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...