Ontology type: schema:ScholarlyArticle
1994-01
AUTHORSYun-Seung Kyung, Madhusudan V. Peshwa, David M. Gryte, Wei-Shou Hu
ABSTRACTIn a continuous culture with cell retention the perfusion rate must be adjusted dynamically to meet the cellular demand. An automated mechanism of adjusting the perfusion rate based on real-time measurement of the metabolic load of the bioreactor is important in achieving a high cell concentration and maintaining high viability. We employed oxygen uptake rate (OUR) measurement as an on-line metabolic indicator of the physiological state of the cells in the bioreactor and adjusted the perfusion rate accordingly. Using an internal hollow fiber microfiltration system for total cell retention, a cell concentration of almost 108 cells/mL was achieved. Although some aggregates were formed during the cultivation, the viability remained high as examined with confocal microscopy after fluorescent vital staining. The results demonstrate that on-line OUR measurement facilitates automated dynamic perfusion and allows a high cell concentration to be achieved. More... »
PAGES183-190
http://scigraph.springernature.com/pub.10.1007/bf00749615
DOIhttp://dx.doi.org/10.1007/bf00749615
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/7765589
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