Ontology type: schema:ScholarlyArticle
1989-02
AUTHORS ABSTRACTConditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses. More... »
PAGES71-74
http://scigraph.springernature.com/pub.10.1007/bf00716841
DOIhttp://dx.doi.org/10.1007/bf00716841
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1045860774
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24232987
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/2103",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Historical Studies",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/21",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "History and Archaeology",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Rothamsted Research",
"id": "https://www.grid.ac/institutes/grid.418374.d",
"name": [
"Department of Biochemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK"
],
"type": "Organization"
},
"familyName": "Lindsey",
"givenName": "K.",
"id": "sg:person.0772622544.88",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772622544.88"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Rothamsted Research",
"id": "https://www.grid.ac/institutes/grid.418374.d",
"name": [
"Department of Biochemistry, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, UK"
],
"type": "Organization"
},
"familyName": "Jones",
"givenName": "M. G. K.",
"id": "sg:person.016427007024.24",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427007024.24"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf00269287",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002663221",
"https://doi.org/10.1007/bf00269287"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269287",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002663221",
"https://doi.org/10.1007/bf00269287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(84)90381-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010023016"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00398663",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021531454",
"https://doi.org/10.1007/bf00398663"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00398663",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021531454",
"https://doi.org/10.1007/bf00398663"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00014185",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335521",
"https://doi.org/10.1007/bf00014185"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00014185",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022335521",
"https://doi.org/10.1007/bf00014185"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3109/10520297209116483",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026274945"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0304-4211(79)90109-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027688455"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00330255",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036019563",
"https://doi.org/10.1007/bf00330255"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00330255",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036019563",
"https://doi.org/10.1007/bf00330255"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269151",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040500001",
"https://doi.org/10.1007/bf00269151"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269151",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040500001",
"https://doi.org/10.1007/bf00269151"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/319791a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045509134",
"https://doi.org/10.1038/319791a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269896",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047809387",
"https://doi.org/10.1007/bf00269896"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269896",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047809387",
"https://doi.org/10.1007/bf00269896"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0168-9452(87)90112-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049904158"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jxb/31.5.1315",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059854948"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2832947",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062564675"
],
"type": "CreativeWork"
}
],
"datePublished": "1989-02",
"datePublishedReg": "1989-02-01",
"description": "Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses. ",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00716841",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1124809",
"issn": [
"0721-7714",
"1432-203X"
],
"name": "Plant Cell Reports",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "8"
}
],
"name": "Stable transformation of sugarbeet protoplasts by electroporation",
"pagination": "71-74",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00716841"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"da74353cb0d779595156e461f5d010fd3487e5ba68b6f1329e393e4e579180eb"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1045860774"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9880970"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"24232987"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00716841",
"https://app.dimensions.ai/details/publication/pub.1045860774"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T09:00",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000375_0000000375/records_91438_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00716841"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00716841'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00716841'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00716841'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00716841'
This table displays all metadata directly associated to this object as RDF triples.
122 TRIPLES
21 PREDICATES
42 URIs
21 LITERALS
9 BLANK NODES