Stable transformation of sugarbeet protoplasts by electroporation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-02

AUTHORS

K. Lindsey, M. G. K. Jones

ABSTRACT

Conditions were optimized for the culture, antibiotic selection and stable transformation by electroporation of suspension culture protoplasts of sugarbeet,Beta vulgaris L.. Highest plating efficiencies (up to 65% at day 21) were obtained if protoplasts were cultured in PGO salts (de Greef and Jacobs, 1979) supplemented with 0.1 mg/1 2,4-D, 0.01 mg/l BAP and 9% mannitol, and in 0.6% agarose rather than in liquid medium. Sensitivity to kanamycin also depended on whether protoplasts were cultured in liquid or agarose medium. Stable transformation of protoplast-derived colonies, as determined by resistance to kanamycin and Southern blot analysis, was achieved by electroporation using both rectangular and exponentially-decaying pulses. More... »

PAGES

71-74

Journal

TITLE

Plant Cell Reports

ISSUE

2

VOLUME

8

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00716841

    DOI

    http://dx.doi.org/10.1007/bf00716841

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1045860774

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24232987


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