Colonic mucins in ulcerative colitis: evidence for loss of sulfation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-10

AUTHORS

Anthony P. Corfield, Neil Myerscough, Niki Bradfield, Clarice Do Amaral Corfield, Margaret Gough, John R. Clamp, Paul Durdey, Bryan F. Warren, David C. C. Bartolo, Kevin R. King, J. Michael Williams

ABSTRACT

Colonic tissue obtained at surgery from control individuals and patients with ulcerative colitis was used to isolate mucins and to prepare mucin glycopolypeptides by pronase digestion. These were compared with mucins labelled with [35S] sulfate and [3H]-glucosamine after organ culture tissue samples from the same patients. A significant loss of mucin sulfation was detected in the colitis patients by both metabolic labelling and chemical analysis of the glycopolypeptides. A change in the size distribution of purified mucin oligosaccharides fractionated on BioGel P6 after release by beta-elimination was seen in both radiolabelled and non-labelled colitis mucins compared with controls. Amino acid analysis of the glycopolypeptides showed a close similarity to the expected ratio of serine:threonine:proline for MUC2 and did not vary between control and colitis groups. Analysis of the mucins confirmed > 90% purity in the labelling experiments, characteristic behaviour on density gradient centrifugation and agarose gel electrophoresis in control and ulcerative colitis groups and differences in sulfation and turnover at various sites in the normal colon. More... »

PAGES

809-822

References to SciGraph publications

  • 1993-01-20. Secreted Mucus Glycoproteins in Cell and Organ Culture in GLYCOPROTEIN ANALYSIS IN BIOMEDICINE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00702345

    DOI

    http://dx.doi.org/10.1007/bf00702345

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048729169

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8910008


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00702345'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00702345'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00702345'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00702345'


     

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    310 https://www.grid.ac/institutes/grid.8348.7 schema:alternateName John Radcliffe Hospital
    311 schema:name Department of Cellular Pathology, John Radcliffe Hospital, OX3 9DU, Headington, Oxford, UK
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