Catalytic properties of Phe41→His mutant of horseradish peroxidase expressed inE. coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-11

AUTHORS

D. B. Loginov, I. G. Gazaryan, V. V. Doseeva, A. G. Galkin, V. I. Tishkov, E. A. Mareeva, M. A. Orlova

ABSTRACT

The recombinant horseradish peroxidase and its single-point F41H mutant have been reactivated fromE. coli inclusion bodies. The influence of the mutation on the heme entrapment, stability and activity of the enzyme was demonstrated. The catalytic rate constants for H2O2 cleavage and ammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) oxidation decrease by two and one orders of magnitude, respectively. Unlike the wild-type recombinant horseradish peroxidase, the elimination of the ABTS oxidation product is not a rate-determining step for the mutant. The F41H replacement results in significant changes of kinetics of iodide ion oxidation. The reaction rate is linear to the concentrations of iodide, H2O2, and the enzyme. The results suggest the direct interaction of iodide with the porphyrin ring of the heme. The decrease in ABTS oxidation activity accompanied by retention of activity in iodide oxidation in the course of low-dosage radiolysis of the F41H mutant is additional evidence of the direct electron transfer from iodide to the heme, in contrast to ABTS oxidation, in which the electron transfer chain in the protein molecule is involved. More... »

PAGES

1923-1927

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00696330

DOI

http://dx.doi.org/10.1007/bf00696330

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045204705


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