Totipotency of tomato protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-05

AUTHORS

E. A. Shahin

ABSTRACT

An efficient and reliable protocol for tomato protoplast isolation, culture, and plant regeneration has been developed. Fourteen diverse cultivars were tested. Fertile plants were regenerated from all 14 cultivars without any modification in the protocol. Plating efficiency (percentage of the protoplasts that formed minicalli) of up to 50% was achieved. Those mini-calli rapidly regenerated shoots at high frequencies. Regenerated shoots can be easily rooted on a basal medium with the appropriate auxin, and have been set to soil within two months after the isolation of the protoplasts. More... »

PAGES

235-240

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00662431

DOI

http://dx.doi.org/10.1007/bf00662431

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033394583

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24253814


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