Restriction fragment length polymorphism analysis of somatic hybrids betweenLycopersicon esculentum and irradiatedL. peruvianum: Evidence for limited donor genome elimination and ... View Full Text


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Article Info

DATE

1990-07

AUTHORS

J. Wijbrandi, P. Zabel, M. Koornneef

ABSTRACT

The genome composition of asymmetric somatic hybrids, obtained by fusion of leaf protoplasts from Lycopersicon esculentum and gamma-irradiated leaf protoplasts from L. peruvianum, was characterised by Southern blot analysis using 29 restriction fragment length polymorphism markers. Eight "low dose hybrids" and seven "high dose hybrids" (irradiation dose 50 Gray and 300 Gray, respectively) were analysed. By densitometric scanning of the autoradiographs, the number of alleles for each locus of the component species was established. In general, elimination of alleles from the irradiated L. peruvianum donor genome was limited and ranged from 17%-69%. Three L. peruvianum loci, located on chromosomes 2, 4 and 7, were present in all asymmetric hybrids, suggesting linkage to the regeneration capacity trait which was used in selecting them. The loss of donor genome was dose-dependent. Low dose hybrids contained more alleles, loci and complete chromosomes from L. peruvianum than high dose hybrids, whereas the high dose hybrids contained more incomplete chromosomes. In most hybrids some L. esculentum alleles were lost. The possible implications of these results for the use of asymmetric hybrids in plant breeding are discussed. More... »

PAGES

270-277

References to SciGraph publications

  • 1988-11. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion” in THEORETICAL AND APPLIED GENETICS
  • 1988. Molecular Mapping of Plant Chromosomes in CHROMOSOME STRUCTURE AND FUNCTION
  • 1987-06. Interspecific T-DNA transfer through plant protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1984. Protoplast Fusion in NONE
  • 1984-10. Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1988-01. Transfer of regeneration capacity from Lycopersiconperuvianum to L.esculentum by protoplast fusion in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-06. Tomato: a crop species amenable to improvement by cellular and molecular methods in EUPHYTICA
  • 1989-02. Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion in THEORETICAL AND APPLIED GENETICS
  • 1990-02. Molecular analysis of the extent of asymmetry in two asymmetric somatic hybrids of tomato in THEORETICAL AND APPLIED GENETICS
  • 1986-02. Variability of DNA content and karyotype in cell cultures of an interdihaploid Solanum tuberosum in PLANT CELL REPORTS
  • 1987-09. A genetic analysis of cell culture traits in tomato in THEORETICAL AND APPLIED GENETICS
  • 1989-03. Preparation of megabase-sized tomato DNA and separation of large restriction fragments by field inversion gel electrophoresis (FIGE) in PLANT MOLECULAR BIOLOGY
  • 1987-10. Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts in THEORETICAL AND APPLIED GENETICS
  • 1989-01. Restriction fragment length polymorphism maps and the concept of graphical genotypes in THEORETICAL AND APPLIED GENETICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 1987-08. X-ray irradiation promoted asymmetric somatic hybridisation and molecular analysis of the products in THEORETICAL AND APPLIED GENETICS
  • 1980-09. Intergeneric gene transfer mediated by plant protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00633828

    DOI

    http://dx.doi.org/10.1007/bf00633828

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1980335


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