Application of statistical design of experiments to the optimization of factor VIII expression by CHO cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-07

AUTHORS

V. Ganne, G. Mignot

ABSTRACT

Selection and optimization of the concentrations of chemically defined components effective on recombinant factor VIII:C production by CHO cells have been performed with statistically designed experiments. Screening of efficient compounds on factor VIII expression was performed by using HADAMARD method, while a precise optimization of the concentrations of two components was achieved with the help of DOEHLERT method. Increased specific activity of factor VIII and reduced cost medium have been obtained with a fewer number of experiments than compared to a less rational approach. More... »

PAGES

233-240

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00624762

DOI

http://dx.doi.org/10.1007/bf00624762

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043258373

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1367666


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