Voltage-dependent properties of macroscopic and elementary calcium channel currents in guinea pig ventricular myocytes View Full Text


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Article Info

DATE

1986-05

AUTHORS

Terence F. McDonald, Adolfo Cavalié, Wolfgang Trautwein, Dieter Pelzer

ABSTRACT

Whole-cell Ca channel currents were recorded from guinea pig ventricular myocytes that were internally perfused with Cs solution and bathed in solutions containing 3.6 mM Ca, 3.6 mM Ba or 90 mM Ba (34° C). Single Ca channel currents were recorded from cell-attached membrane patches of similar myocytes; the patch pipettes contained a 90 mM Ba solution. 1. Although the shape of the whole-cellI–V relation was independent of the bathing solution, this was not the case with the location of the inward current maximum (Vpeak);Vpeak in 90 mM Ba was about 30 mV positive toVpeak in 3.6 mM Ba. 2. The activation and inactivation of whole-cell currents were voltage dependent. Compared to the voltage dependencies in 3.6 mM Ba, those in 90 mM Ba were shifted by about 30 mV to the right, suggesting a neutralization of surface charges. 3. Observations compatible with the ion permeation model proposed by Hess and Tsien (1984) included (a) a depression of current during Ca/Ba solution exchange, (b) a high divalent to monovalent ion permeability, and (c) rectification of the outward limb of theI–V relation. 4. Estimated current densities atVpeak were similar for myocytes in 3.6 mM Ca and 3.6 mM Ba, and about 10 times larger in 90 mM Ba. 5. Average currents (I*) calculated from ensembles of records of single Ca channel current had voltage-dependent time courses resembling those of whole-cellIBa (90 mM). 6. Single-channelI*–V relations were superimposable on whole-cellI–V curves suggesting that voltage-dependent single-channel parameters (probability of opening, elementary current amplitude) can be related to the voltage-dependent macroscopic current parameters (activation, instantaneousI–V relation) when scaled by channel number. 7. The density of Ca channels in myocytes was calculated from whole-cellIBa (90 mM) and average current through single channels. The outcome, 3–5 channels/μm2, agrees with two other recent estimates (Tsien et al. 1983; Lux and Brown 1984). However, it is difficult to reconcile with the much lower density that one would forecast from the frequency of functional channel observation in myocyte membrane patches (Pelzer et al. 1985c). More... »

PAGES

437-448

References to SciGraph publications

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  • 1982-10. Calcium currents of isolated bovine ventricular myocytes are fast and of large amplitude in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1975-03. Calcium conductance and tension in mammalian ventricular muscle in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1984-02. Low resting potentials in single isolated heart cells due to membrane damage by the recording microelectrode in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1986. Activation and Inactivation of Single Calcium Channels in Cardiac Cells in CALCIUM ELECTROGENESIS AND NEURONAL FUNCTIONING
  • 1981-08. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1985-10. Temperature-induced transitory and steady-state changes in the calcium current of guinea pig ventricular myocytes in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1985-05. Removal of Ca current inactivation in dialysed guinea-pig atrial cardioballs by Ca chelators in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1983-02. Interaction between calcium ions and surface charge as it relates to calcium currents in THE JOURNAL OF MEMBRANE BIOLOGY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00583365

    DOI

    http://dx.doi.org/10.1007/bf00583365

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1032458642

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2423956


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    36 schema:description Whole-cell Ca channel currents were recorded from guinea pig ventricular myocytes that were internally perfused with Cs solution and bathed in solutions containing 3.6 mM Ca, 3.6 mM Ba or 90 mM Ba (34° C). Single Ca channel currents were recorded from cell-attached membrane patches of similar myocytes; the patch pipettes contained a 90 mM Ba solution. 1. Although the shape of the whole-cellI–V relation was independent of the bathing solution, this was not the case with the location of the inward current maximum (Vpeak);Vpeak in 90 mM Ba was about 30 mV positive toVpeak in 3.6 mM Ba. 2. The activation and inactivation of whole-cell currents were voltage dependent. Compared to the voltage dependencies in 3.6 mM Ba, those in 90 mM Ba were shifted by about 30 mV to the right, suggesting a neutralization of surface charges. 3. Observations compatible with the ion permeation model proposed by Hess and Tsien (1984) included (a) a depression of current during Ca/Ba solution exchange, (b) a high divalent to monovalent ion permeability, and (c) rectification of the outward limb of theI–V relation. 4. Estimated current densities atVpeak were similar for myocytes in 3.6 mM Ca and 3.6 mM Ba, and about 10 times larger in 90 mM Ba. 5. Average currents (I*) calculated from ensembles of records of single Ca channel current had voltage-dependent time courses resembling those of whole-cellIBa (90 mM). 6. Single-channelI*–V relations were superimposable on whole-cellI–V curves suggesting that voltage-dependent single-channel parameters (probability of opening, elementary current amplitude) can be related to the voltage-dependent macroscopic current parameters (activation, instantaneousI–V relation) when scaled by channel number. 7. The density of Ca channels in myocytes was calculated from whole-cellIBa (90 mM) and average current through single channels. The outcome, 3–5 channels/μm2, agrees with two other recent estimates (Tsien et al. 1983; Lux and Brown 1984). However, it is difficult to reconcile with the much lower density that one would forecast from the frequency of functional channel observation in myocyte membrane patches (Pelzer et al. 1985c).
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