Inhibition of human immunodeficiency virus replication in a human T cell line by antisense RNA expressed in the cell View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-07

AUTHORS

Jun-Ichiro Gyotoku, Mohamed A. El-Farrash, Shinji Fujimoto, Wilfred T. V. Germeraad, Yoshihiko Watanabe, Keisuke Teshigawara, Shinji Harada, Yoshimoto Katsura

ABSTRACT

The effect of the expression of antisense RNA against the human immunodeficiency virus (HIV) genome in a human T-cell line CEM on HIV replication was investigated. A 2.7 kilobase (kb) fragment of the HIV genome, includingtat and a part ofrev andenv, was cloned into the retroviral vector WB in the antisense orientation under the SV40 or H-2K promoter. CEM cells transduced with this antisense gene via recombinant retrovirus expressed the RNAs of three different molecular sizes containing the antisense construct. CEM cells and these transduced cells were infected with HIV. HIV replication was evaluated 4–10 days later by an immunofluorescense assay and by determining the reverse transcriptase activity in the culture supernatant. The results indicate that although the recombinant retrovirus WB strongly enhanced the HIV replication in CEM cells, the expression of antisense RNA in the cells was highly effective in impeding the replication of HIV. The inhibitory effect was especially high in CEM cells transduced with the antisense gene under the control of SV40 promoter. In this case, HIV antigen-positive cells and reverse transcriptase activity in the culture supernatant of transduced cells were reduced to 30–50% and 5–10% of those in CEM cells and in the CEM cells transduced with WB, respectively. More... »

PAGES

189-202

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00568969

DOI

http://dx.doi.org/10.1007/bf00568969

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018115096

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1722929


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