Skeletal muscle cell populations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-01

AUTHORS

Z. Yablonka-Reuveni, M. Nameroff

ABSTRACT

Cell suspensions from the breast muscles of 10-day old chicken embryos were separated into non-myogenic, fibroblast-like cell fractions and a mononucleated, myogenic cell fraction by Percoll density centrifugation. Isolated populations were characterized by their morphology in both mass cultures and individual macroscopic clones and by the immunocytochemical detection of skeletal muscle- and smooth muscle-specific proteins in individual cells. Cell populations were also characterized by their protein patterns using sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The less dense, non-myogenic cells comprised 16% of the cells. In culture they were predominantly flattened, stellate cells and gave rise to clones lacking myotubes. These fibroblast-like cells were negative for skeletal muscle myosin or muscle type creatine phosphokinase. Less than 0.1% of these cells demonstrated strong fluorescence when stained with anti-desmin or anti-smooth muscle specific actin. This observation suggested that the vast majority of these cells were not related to vascular smooth muscle cells. Also, over 99% of the non-myogenic cells did not display characteristic properties of endothelial cells. The denser myogenic cell fraction comprised over 80% of the cells and in clonal cultures gave rise to about 70% myogenic clones. An additional 30% of clones from this fraction were non-myogenic indicating heterogeneity in this population. We conclude that Percoll centrifugation can be employed for the isolation of myogenic and non-myogenic cell populations directly from the embryonic muscle. Moreover, this procedure allows the direct analysis of cell-specific proteins (e.g., by gel electrophoresis) without the need for cell culturing. The results thus obtained closely reflect the status of the cells in the intact muscle. More... »

PAGES

27-38

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00518721

DOI

http://dx.doi.org/10.1007/bf00518721

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029911424

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3038797


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Cycle", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Separation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Centrifugation, Density Gradient", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chick Embryo", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Desmin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fibroblasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunologic Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscle, Smooth", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Povidone", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Silicon Dioxide", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, School of Medicine, University of Washington, 98195, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yablonka-Reuveni", 
        "givenName": "Z.", 
        "id": "sg:person.01353366552.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353366552.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Washington", 
          "id": "https://www.grid.ac/institutes/grid.34477.33", 
          "name": [
            "Department of Biological Structure, School of Medicine, University of Washington, 98195, Seattle, Washington, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nameroff", 
        "givenName": "M.", 
        "id": "sg:person.0613762002.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613762002.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.55.1.119", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004043087"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-119101-6.50010-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006008077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.102.3.740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006916870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(71)90437-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009954178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1983.tb01310.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009982765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0070-2153(08)60480-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011454994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.100.3.807", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013744745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/278067a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014630165", 
          "https://doi.org/10.1038/278067a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/278067a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014630165", 
          "https://doi.org/10.1038/278067a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.51.070182.001251", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015542557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(81)90183-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015951429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(81)90183-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015951429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(75)90405-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016253781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(82)90034-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018579086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(77)90108-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022338820"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(87)90226-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027959411"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1984.tb00279.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028028372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(84)90311-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028351921"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.96.2.401", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028972531"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(80)90077-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030608580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(80)90077-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030608580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1984.tb01383.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030694266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1979.tb00991.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031155919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.71.4.1506", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033065597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(77)90311-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036032276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(74)90154-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039602221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00296451", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039885759", 
          "https://doi.org/10.1007/bf00296451"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.76.8.3894", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042522693"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0012-1606(79)90006-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042979538"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0012-1606(81)80010-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043113533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(84)90693-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043682904"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.99.6.2034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045480839"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/313692a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048412074", 
          "https://doi.org/10.1038/313692a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1978.tb00949.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049498788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-0436.1979.tb00992.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052405547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.140.3573.1273", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062480178"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074792077", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080097711", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081065851", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081693803", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081929524", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082123851", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1987-01", 
    "datePublishedReg": "1987-01-01", 
    "description": "Cell suspensions from the breast muscles of 10-day old chicken embryos were separated into non-myogenic, fibroblast-like cell fractions and a mononucleated, myogenic cell fraction by Percoll density centrifugation. Isolated populations were characterized by their morphology in both mass cultures and individual macroscopic clones and by the immunocytochemical detection of skeletal muscle- and smooth muscle-specific proteins in individual cells. Cell populations were also characterized by their protein patterns using sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The less dense, non-myogenic cells comprised 16% of the cells. In culture they were predominantly flattened, stellate cells and gave rise to clones lacking myotubes. These fibroblast-like cells were negative for skeletal muscle myosin or muscle type creatine phosphokinase. Less than 0.1% of these cells demonstrated strong fluorescence when stained with anti-desmin or anti-smooth muscle specific actin. This observation suggested that the vast majority of these cells were not related to vascular smooth muscle cells. Also, over 99% of the non-myogenic cells did not display characteristic properties of endothelial cells. The denser myogenic cell fraction comprised over 80% of the cells and in clonal cultures gave rise to about 70% myogenic clones. An additional 30% of clones from this fraction were non-myogenic indicating heterogeneity in this population. We conclude that Percoll centrifugation can be employed for the isolation of myogenic and non-myogenic cell populations directly from the embryonic muscle. Moreover, this procedure allows the direct analysis of cell-specific proteins (e.g., by gel electrophoresis) without the need for cell culturing. The results thus obtained closely reflect the status of the cells in the intact muscle.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00518721", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2462468", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2460959", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "87"
      }
    ], 
    "name": "Skeletal muscle cell populations", 
    "pagination": "27-38", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8e7ecb434ce864670ce0a99186b8a0890c991c491f8e76296b92ab013705ba64"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "3038797"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0411300"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00518721"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029911424"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00518721", 
      "https://app.dimensions.ai/details/publication/pub.1029911424"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:02", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130831_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00518721"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00518721'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00518721'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00518721'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00518721'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      84 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00518721 schema:about N07e3395f2b8140e5a5606d263696ef20
2 N26addfde4d434969b8379f5d534882dd
3 N28c32949ff3c46708164663891ed4838
4 N33a025830628486d9e2f071f25c24272
5 N481820dbb258422bbeefa680c84e59dc
6 N5e663fbb4ec6438db112641b9ef179c9
7 N645ab77327194facb2660326d863bed0
8 N75ff3722dc9c4cd29f4dfc0bb8192641
9 N7c78b7686dc44d2fad2cf0d31bf9e4b1
10 N901887364898401cb9891960be91cc01
11 N902082710e974c409f2811c72282280f
12 N9eb6e9fb0aac4e39b4e78628cdaf0a05
13 Nc3df0755af904858bc761fec98f03740
14 Nc763f73ecea3480ab5969d1f955de72d
15 Nfbf7aa8f196242e9b440fa3ee9dea844
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N20e8037e7dc74a0caba5878ef71375e2
19 schema:citation sg:pub.10.1007/bf00296451
20 sg:pub.10.1038/227680a0
21 sg:pub.10.1038/278067a0
22 sg:pub.10.1038/313692a0
23 https://app.dimensions.ai/details/publication/pub.1074792077
24 https://app.dimensions.ai/details/publication/pub.1080097711
25 https://app.dimensions.ai/details/publication/pub.1081065851
26 https://app.dimensions.ai/details/publication/pub.1081693803
27 https://app.dimensions.ai/details/publication/pub.1081929524
28 https://app.dimensions.ai/details/publication/pub.1082123851
29 https://doi.org/10.1016/0012-1606(74)90154-7
30 https://doi.org/10.1016/0012-1606(75)90405-4
31 https://doi.org/10.1016/0012-1606(77)90108-7
32 https://doi.org/10.1016/0012-1606(79)90006-x
33 https://doi.org/10.1016/0012-1606(87)90226-0
34 https://doi.org/10.1016/0014-4827(71)90437-x
35 https://doi.org/10.1016/0014-4827(77)90311-1
36 https://doi.org/10.1016/0014-4827(82)90034-9
37 https://doi.org/10.1016/0014-4827(84)90693-1
38 https://doi.org/10.1016/0022-1759(80)90077-0
39 https://doi.org/10.1016/0022-1759(81)90183-6
40 https://doi.org/10.1016/0092-8674(84)90311-8
41 https://doi.org/10.1016/b978-0-12-119101-6.50010-x
42 https://doi.org/10.1016/s0012-1606(81)80010-3
43 https://doi.org/10.1016/s0070-2153(08)60480-9
44 https://doi.org/10.1073/pnas.55.1.119
45 https://doi.org/10.1073/pnas.71.4.1506
46 https://doi.org/10.1073/pnas.76.8.3894
47 https://doi.org/10.1083/jcb.100.3.807
48 https://doi.org/10.1083/jcb.102.3.740
49 https://doi.org/10.1083/jcb.96.2.401
50 https://doi.org/10.1083/jcb.99.6.2034
51 https://doi.org/10.1111/j.1432-0436.1978.tb00949.x
52 https://doi.org/10.1111/j.1432-0436.1979.tb00991.x
53 https://doi.org/10.1111/j.1432-0436.1979.tb00992.x
54 https://doi.org/10.1111/j.1432-0436.1983.tb01310.x
55 https://doi.org/10.1111/j.1432-0436.1984.tb00279.x
56 https://doi.org/10.1111/j.1432-0436.1984.tb01383.x
57 https://doi.org/10.1126/science.140.3573.1273
58 https://doi.org/10.1146/annurev.bi.51.070182.001251
59 schema:datePublished 1987-01
60 schema:datePublishedReg 1987-01-01
61 schema:description Cell suspensions from the breast muscles of 10-day old chicken embryos were separated into non-myogenic, fibroblast-like cell fractions and a mononucleated, myogenic cell fraction by Percoll density centrifugation. Isolated populations were characterized by their morphology in both mass cultures and individual macroscopic clones and by the immunocytochemical detection of skeletal muscle- and smooth muscle-specific proteins in individual cells. Cell populations were also characterized by their protein patterns using sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The less dense, non-myogenic cells comprised 16% of the cells. In culture they were predominantly flattened, stellate cells and gave rise to clones lacking myotubes. These fibroblast-like cells were negative for skeletal muscle myosin or muscle type creatine phosphokinase. Less than 0.1% of these cells demonstrated strong fluorescence when stained with anti-desmin or anti-smooth muscle specific actin. This observation suggested that the vast majority of these cells were not related to vascular smooth muscle cells. Also, over 99% of the non-myogenic cells did not display characteristic properties of endothelial cells. The denser myogenic cell fraction comprised over 80% of the cells and in clonal cultures gave rise to about 70% myogenic clones. An additional 30% of clones from this fraction were non-myogenic indicating heterogeneity in this population. We conclude that Percoll centrifugation can be employed for the isolation of myogenic and non-myogenic cell populations directly from the embryonic muscle. Moreover, this procedure allows the direct analysis of cell-specific proteins (e.g., by gel electrophoresis) without the need for cell culturing. The results thus obtained closely reflect the status of the cells in the intact muscle.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree false
65 schema:isPartOf N2378d4b95a294ff186ee10cce638d68a
66 N5f1b845c309746f1b8c52351409b4b6a
67 sg:journal.1294817
68 schema:name Skeletal muscle cell populations
69 schema:pagination 27-38
70 schema:productId N5178412ea54f4ce8a7b2b3aac7efb6f0
71 N7b756a47ef884f07a8567865ecfeaa0a
72 Na84f4b00061442dc966e559633d9400e
73 Naa40370c365b48a9adcc38c63483e635
74 Nbe5b6e18cd6346a19f3c13ce20fbb333
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029911424
76 https://doi.org/10.1007/bf00518721
77 schema:sdDatePublished 2019-04-11T14:02
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher Nc6f61028073a43de92bde85ffa2491c4
80 schema:url http://link.springer.com/10.1007/BF00518721
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N07e3395f2b8140e5a5606d263696ef20 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Cell Cycle
86 rdf:type schema:DefinedTerm
87 N20e8037e7dc74a0caba5878ef71375e2 rdf:first sg:person.01353366552.13
88 rdf:rest Nfbc930de2bc341f5b5f60917cfc33128
89 N2378d4b95a294ff186ee10cce638d68a schema:issueNumber 1
90 rdf:type schema:PublicationIssue
91 N26addfde4d434969b8379f5d534882dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Cells, Cultured
93 rdf:type schema:DefinedTerm
94 N28c32949ff3c46708164663891ed4838 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
95 schema:name Centrifugation, Density Gradient
96 rdf:type schema:DefinedTerm
97 N33a025830628486d9e2f071f25c24272 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cell Separation
99 rdf:type schema:DefinedTerm
100 N481820dbb258422bbeefa680c84e59dc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Muscle, Smooth
102 rdf:type schema:DefinedTerm
103 N5178412ea54f4ce8a7b2b3aac7efb6f0 schema:name pubmed_id
104 schema:value 3038797
105 rdf:type schema:PropertyValue
106 N5e663fbb4ec6438db112641b9ef179c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Muscle Proteins
108 rdf:type schema:DefinedTerm
109 N5f1b845c309746f1b8c52351409b4b6a schema:volumeNumber 87
110 rdf:type schema:PublicationVolume
111 N645ab77327194facb2660326d863bed0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Muscles
113 rdf:type schema:DefinedTerm
114 N75ff3722dc9c4cd29f4dfc0bb8192641 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Immunologic Techniques
116 rdf:type schema:DefinedTerm
117 N7b756a47ef884f07a8567865ecfeaa0a schema:name nlm_unique_id
118 schema:value 0411300
119 rdf:type schema:PropertyValue
120 N7c78b7686dc44d2fad2cf0d31bf9e4b1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Silicon Dioxide
122 rdf:type schema:DefinedTerm
123 N901887364898401cb9891960be91cc01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Desmin
125 rdf:type schema:DefinedTerm
126 N902082710e974c409f2811c72282280f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Actins
128 rdf:type schema:DefinedTerm
129 N9eb6e9fb0aac4e39b4e78628cdaf0a05 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Fibroblasts
131 rdf:type schema:DefinedTerm
132 Na84f4b00061442dc966e559633d9400e schema:name doi
133 schema:value 10.1007/bf00518721
134 rdf:type schema:PropertyValue
135 Naa40370c365b48a9adcc38c63483e635 schema:name dimensions_id
136 schema:value pub.1029911424
137 rdf:type schema:PropertyValue
138 Nbe5b6e18cd6346a19f3c13ce20fbb333 schema:name readcube_id
139 schema:value 8e7ecb434ce864670ce0a99186b8a0890c991c491f8e76296b92ab013705ba64
140 rdf:type schema:PropertyValue
141 Nc3df0755af904858bc761fec98f03740 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Povidone
143 rdf:type schema:DefinedTerm
144 Nc6f61028073a43de92bde85ffa2491c4 schema:name Springer Nature - SN SciGraph project
145 rdf:type schema:Organization
146 Nc763f73ecea3480ab5969d1f955de72d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Chick Embryo
148 rdf:type schema:DefinedTerm
149 Nfbc930de2bc341f5b5f60917cfc33128 rdf:first sg:person.0613762002.05
150 rdf:rest rdf:nil
151 Nfbf7aa8f196242e9b440fa3ee9dea844 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Animals
153 rdf:type schema:DefinedTerm
154 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
155 schema:name Biological Sciences
156 rdf:type schema:DefinedTerm
157 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
158 schema:name Biochemistry and Cell Biology
159 rdf:type schema:DefinedTerm
160 sg:grant.2460959 http://pending.schema.org/fundedItem sg:pub.10.1007/bf00518721
161 rdf:type schema:MonetaryGrant
162 sg:grant.2462468 http://pending.schema.org/fundedItem sg:pub.10.1007/bf00518721
163 rdf:type schema:MonetaryGrant
164 sg:journal.1294817 schema:issn 0948-6143
165 1432-119X
166 schema:name Histochemistry and Cell Biology
167 rdf:type schema:Periodical
168 sg:person.01353366552.13 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
169 schema:familyName Yablonka-Reuveni
170 schema:givenName Z.
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01353366552.13
172 rdf:type schema:Person
173 sg:person.0613762002.05 schema:affiliation https://www.grid.ac/institutes/grid.34477.33
174 schema:familyName Nameroff
175 schema:givenName M.
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0613762002.05
177 rdf:type schema:Person
178 sg:pub.10.1007/bf00296451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039885759
179 https://doi.org/10.1007/bf00296451
180 rdf:type schema:CreativeWork
181 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
182 https://doi.org/10.1038/227680a0
183 rdf:type schema:CreativeWork
184 sg:pub.10.1038/278067a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014630165
185 https://doi.org/10.1038/278067a0
186 rdf:type schema:CreativeWork
187 sg:pub.10.1038/313692a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048412074
188 https://doi.org/10.1038/313692a0
189 rdf:type schema:CreativeWork
190 https://app.dimensions.ai/details/publication/pub.1074792077 schema:CreativeWork
191 https://app.dimensions.ai/details/publication/pub.1080097711 schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1081065851 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1081693803 schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1081929524 schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1082123851 schema:CreativeWork
196 https://doi.org/10.1016/0012-1606(74)90154-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039602221
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/0012-1606(75)90405-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016253781
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/0012-1606(77)90108-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022338820
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/0012-1606(79)90006-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1042979538
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0012-1606(87)90226-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027959411
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0014-4827(71)90437-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009954178
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0014-4827(77)90311-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036032276
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/0014-4827(82)90034-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018579086
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/0014-4827(84)90693-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043682904
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/0022-1759(80)90077-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030608580
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/0022-1759(81)90183-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015951429
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/0092-8674(84)90311-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028351921
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/b978-0-12-119101-6.50010-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006008077
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/s0012-1606(81)80010-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043113533
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/s0070-2153(08)60480-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011454994
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1073/pnas.55.1.119 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004043087
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1073/pnas.71.4.1506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033065597
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1073/pnas.76.8.3894 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042522693
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1083/jcb.100.3.807 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013744745
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1083/jcb.102.3.740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006916870
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1083/jcb.96.2.401 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028972531
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1083/jcb.99.6.2034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045480839
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1111/j.1432-0436.1978.tb00949.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1049498788
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1111/j.1432-0436.1979.tb00991.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031155919
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1111/j.1432-0436.1979.tb00992.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052405547
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1111/j.1432-0436.1983.tb01310.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009982765
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1111/j.1432-0436.1984.tb00279.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1028028372
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1111/j.1432-0436.1984.tb01383.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030694266
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1126/science.140.3573.1273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062480178
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1146/annurev.bi.51.070182.001251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015542557
255 rdf:type schema:CreativeWork
256 https://www.grid.ac/institutes/grid.34477.33 schema:alternateName University of Washington
257 schema:name Department of Biological Structure, School of Medicine, University of Washington, 98195, Seattle, Washington, USA
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...