The influence of fixation and tissue preparation on the immunohistochemical demonstration of fibronectin in human tissue View Full Text


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Article Info

DATE

1981-06

AUTHORS

B. Hølund, P. P. Clausen, I. Clemmensen

ABSTRACT

The influence of fixation and tissue preparation on the immunohistochemical localization of human fibronectin in gastrointestinal tract tissue has been examined using indirect immunoperoxidase technique. The most optimal staining result with strong intensity and well defined localization was obtained on frozen sections of unfixed material. Nearly identical results with improved morphology were obtained when staining paraffin sections of tissue fixed in 96% ethanol, 96%+1% acetic acid and absolute acetone. All other fixatives tested, 10% neutral buffered formalin, Lillie's AAF, Bouin's fixative, Clarke's fixative, 4% formaldehyde, 4% formaldehyde+ 0.5% cetylpyridiniumchloride (F-CPC), 4% formaldehyde +0.1% glutaraldehyde gave unsatisfactory results. However, proteolytic digestion with pepsin of paraffin sections prior to staining of buffered formalin and F-CPCfixed material gave results comparable with those obtained on unfixed frozen sections as regards definition of the staining whereas staining intensity was decreased in some degree. No improvement was observed when using proteolytic digestion of tissue fixed in other fixatives. More... »

PAGES

291-299

References to SciGraph publications

  • 1979-11. Fibronectin distribution in epithelial and associated tissues of the rat in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1979-08. Studies on fibronectin in the skin in ARCHIVES OF DERMATOLOGICAL RESEARCH
  • 1980-01. Immunoperoxidase localisation of fibronectin in glomeruli of formalin fixed paraffin processed renal tissue in HISTOCHEMISTRY AND CELL BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00517142

    DOI

    http://dx.doi.org/10.1007/bf00517142

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1020244230

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6168612


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