Streptomyces rimosus extracellular proteases View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-09

AUTHORS

M. Renko, M. Pokorny, Lj. Vitale, V. Turk

ABSTRACT

A procedure for purification of a serine alkaline proteinase from Streptomyces rimosus waste broth was developed. The procedure includes ultrafiltration, CM-Sephadex C-50 and CM-cellulose chromatography and gel filtration on Sephadex G-75. The recovery of electrophoretically pure enzyme was 25%. The enzyme is active on different protein and synthetic substrates at neutral and alkaline pHs but its activity on hemoglobin and bovine serum albumin, is optimal at pH 4.0–4.5. It has a molecular weight of 22,000 and a pI of 4.90. The enzyme is inhibited by DFP and PMSF but not by TPCK. Its substrate specificity, amino acid composition and some other properties were also determined. More... »

PAGES

166-171

References to SciGraph publications

  • 1979-03. Streptomyces rimosus extracellular proteases in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00511256

    DOI

    http://dx.doi.org/10.1007/bf00511256

    DIMENSIONS

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