Improved section adhesion for immunocytochemistry using high molecular weight polymers of l-lysine as a slide coating View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-06

AUTHORS

W. M. Huang, S. J. Gibson, P. Facer, J. Gu, J. M. Polak

ABSTRACT

Poly-l-lysine (PPL) has been used to coat glass slides in the preparation of tissue sections for immunocytochemical staining. The adhesive properties of different molecular weight (m.w.) polymers of l-lysine have been tested on pre-fixed cryostat sections which were subjected to a 3 day washing treatment. It has been found that the higher the molecular weight of the polymer, the greater the adhesive force it provides. PLL (m.w. 350,000) at concentrations in the range of 0.05–0.1% was found to be the most effective polymer. More... »

PAGES

275-279

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00506570

DOI

http://dx.doi.org/10.1007/bf00506570

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048621460

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6188729


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