Medium optimization for a methanol utilizing bacterium based on chemostat theory View Full Text


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Article Info

DATE

1980-06

AUTHORS

Yoshinobu Tsuchiya, Naomichi Nishio, Shiro Nagai

ABSTRACT

In the productions of biomass and vitamin B12 using methanol as the sole carbon source, it is necessary to use a medium in which methanol is the growth limiting substrate. Other inorganic salts should be in slight excess so that the yield of cells and the intracellular content of vitamin B12 do not vary. From basic principles of chemostat culture, a medium was optimized for Pseudomonas AM-1 a methanol utilizing bacterium, for the concentrations of various inorganic salts. This was done in a series of chemostat cultures at a dilution rate of 0.1 h−1. Optimum amounts of NH4+, PO43- and Mg2+ were estimated from the minimum concentration of the salt at which methanol became growth limiting. The optimum concentrations of Ca2+, Fe2+, Mn2+, and Zn2+ as a group were determined in the same way. Cu2+, Mo6+, Co2+ and B3+ are required at concentrations of μg/l and they were not studied as these very low level can be introduced as contaminants from other salts. The optimum medium composition (in g/l) was as follows: (NH4)2SO4, 1.0; H3PO4, 75×10−3; MgSO4 · 7H2O, 30×10−3; CaCl2 · 2H2O, 3.3×10−3; FeSO4 · 7H2O, 1.3×10−3, MnSO4 · 4H2O, 0.13×10−3; ZnSO4 · 7H2O, 0.13×10−3; CuSO4 · 5H2O, 40×10−6; Na2MoO4, 40×10−6; CoCl2 · 6H2O, 40×10−6; H3BO3, 30×10−6 and methanol 4. More... »

PAGES

121-127

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00503507

DOI

http://dx.doi.org/10.1007/bf00503507

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030302172


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