Diffusion of oxygen in alginate gels related to the kinetics of methanol oxidation by immobilized Hansenula polymorpha cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1983-07

AUTHORS

Harry Hiemstra, Lubbert Dijkhuizen, Wim Harder

ABSTRACT

In the yeast Hansenula polymorpha an oxygen-requiring enzyme, alcohol oxidase, catalyzes the conversion of methanol into formaldehyde. After growth on methanol cells of the organism were harvested and entrapped in barium-alginate gels. The diffusion of oxygen towards these cells is seriously hindered by the polymer network. This caused significant changes in the kinetics of methanol oxidation by the immobilized cells as compared to that observed with cells free in suspension. The apparent KS(O2) of the immobilized cells was dependent upon the density of the cells in the alginate beads and the bead radius. Using a well-known theoretical model, originally developed to describe the kinetics of oxygen diffusion in mold pellets, the diffusion coefficient for oxygen in barium-alginate gels was estimated. This coefficient was only 25% of that in water. The model also allowed an adequate simulation of the observed kinetics of methanol oxidation by the immobilized cells. More... »

PAGES

189-196

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00501507

DOI

http://dx.doi.org/10.1007/bf00501507

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008570596


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hiemstra", 
        "givenName": "Harry", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dijkhuizen", 
        "givenName": "Lubbert", 
        "id": "sg:person.01322127264.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322127264.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Harder", 
        "givenName": "Wim", 
        "id": "sg:person.01033753364.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033753364.95"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00505015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015922032", 
          "https://doi.org/10.1007/bf00505015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00505015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015922032", 
          "https://doi.org/10.1007/bf00505015"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00703041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021068553", 
          "https://doi.org/10.1007/bf00703041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00703041", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021068553", 
          "https://doi.org/10.1007/bf00703041"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-5193(73)90153-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021209388"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00446560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033607761", 
          "https://doi.org/10.1007/bf00446560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00446560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033607761", 
          "https://doi.org/10.1007/bf00446560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(76)44031-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033803130"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00510565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034361920", 
          "https://doi.org/10.1007/bf00510565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00510565", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034361920", 
          "https://doi.org/10.1007/bf00510565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260211202", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040101312"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260230713", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046008729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260181111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050266771"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260130412", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051326196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260220605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052647630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260230905", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053657003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00498724", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053723453", 
          "https://doi.org/10.1007/bf00498724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00498724", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053723453", 
          "https://doi.org/10.1007/bf00498724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075763875", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1983-07", 
    "datePublishedReg": "1983-07-01", 
    "description": "In the yeast Hansenula polymorpha an oxygen-requiring enzyme, alcohol oxidase, catalyzes the conversion of methanol into formaldehyde. After growth on methanol cells of the organism were harvested and entrapped in barium-alginate gels. The diffusion of oxygen towards these cells is seriously hindered by the polymer network. This caused significant changes in the kinetics of methanol oxidation by the immobilized cells as compared to that observed with cells free in suspension. The apparent KS(O2) of the immobilized cells was dependent upon the density of the cells in the alginate beads and the bead radius. Using a well-known theoretical model, originally developed to describe the kinetics of oxygen diffusion in mold pellets, the diffusion coefficient for oxygen in barium-alginate gels was estimated. This coefficient was only 25% of that in water. The model also allowed an adequate simulation of the observed kinetics of methanol oxidation by the immobilized cells.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00501507", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "18"
      }
    ], 
    "name": "Diffusion of oxygen in alginate gels related to the kinetics of methanol oxidation by immobilized Hansenula polymorpha cells", 
    "pagination": "189-196", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "28d5065b00ddb3e6f24e55dc6abb512f98429e7f76239783d7fe461ed756703f"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00501507"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1008570596"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00501507", 
      "https://app.dimensions.ai/details/publication/pub.1008570596"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130793_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00501507"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00501507'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00501507'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00501507'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00501507'


 

This table displays all metadata directly associated to this object as RDF triples.

120 TRIPLES      21 PREDICATES      41 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00501507 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N2ea9fb511be34daf83ca9e1af3016c48
4 schema:citation sg:pub.10.1007/bf00446560
5 sg:pub.10.1007/bf00498724
6 sg:pub.10.1007/bf00505015
7 sg:pub.10.1007/bf00510565
8 sg:pub.10.1007/bf00703041
9 https://app.dimensions.ai/details/publication/pub.1075763875
10 https://doi.org/10.1002/bit.260130412
11 https://doi.org/10.1002/bit.260181111
12 https://doi.org/10.1002/bit.260211202
13 https://doi.org/10.1002/bit.260220605
14 https://doi.org/10.1002/bit.260230713
15 https://doi.org/10.1002/bit.260230905
16 https://doi.org/10.1016/0022-5193(73)90153-7
17 https://doi.org/10.1016/s0076-6879(76)44031-4
18 schema:datePublished 1983-07
19 schema:datePublishedReg 1983-07-01
20 schema:description In the yeast Hansenula polymorpha an oxygen-requiring enzyme, alcohol oxidase, catalyzes the conversion of methanol into formaldehyde. After growth on methanol cells of the organism were harvested and entrapped in barium-alginate gels. The diffusion of oxygen towards these cells is seriously hindered by the polymer network. This caused significant changes in the kinetics of methanol oxidation by the immobilized cells as compared to that observed with cells free in suspension. The apparent KS(O2) of the immobilized cells was dependent upon the density of the cells in the alginate beads and the bead radius. Using a well-known theoretical model, originally developed to describe the kinetics of oxygen diffusion in mold pellets, the diffusion coefficient for oxygen in barium-alginate gels was estimated. This coefficient was only 25% of that in water. The model also allowed an adequate simulation of the observed kinetics of methanol oxidation by the immobilized cells.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree true
24 schema:isPartOf N6f6843857af147eb860ff49ccdc843d6
25 Nae34e0721f4a4e12a36aa765daf7f4b6
26 sg:journal.1083533
27 schema:name Diffusion of oxygen in alginate gels related to the kinetics of methanol oxidation by immobilized Hansenula polymorpha cells
28 schema:pagination 189-196
29 schema:productId N7ac1836713114c6c83dc90a5d7d7cd56
30 N81bb6dcd6a9346078e195e1581d6922c
31 Na7cf78a6e80f4e8abb9f4fdbbb9743ff
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008570596
33 https://doi.org/10.1007/bf00501507
34 schema:sdDatePublished 2019-04-11T13:48
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher N90430995aa0d42d0b71b50bae28ce0df
37 schema:url http://link.springer.com/10.1007/BF00501507
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N2ea9fb511be34daf83ca9e1af3016c48 rdf:first N3b1795c309c042689ffe88362f26a6b6
42 rdf:rest N56cf09a6cad449e49fad15e9fbd276fe
43 N3b1795c309c042689ffe88362f26a6b6 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
44 schema:familyName Hiemstra
45 schema:givenName Harry
46 rdf:type schema:Person
47 N56cf09a6cad449e49fad15e9fbd276fe rdf:first sg:person.01322127264.74
48 rdf:rest Nee10197faf8e4de2b88630a15cf51b69
49 N6f6843857af147eb860ff49ccdc843d6 schema:volumeNumber 18
50 rdf:type schema:PublicationVolume
51 N7ac1836713114c6c83dc90a5d7d7cd56 schema:name doi
52 schema:value 10.1007/bf00501507
53 rdf:type schema:PropertyValue
54 N81bb6dcd6a9346078e195e1581d6922c schema:name dimensions_id
55 schema:value pub.1008570596
56 rdf:type schema:PropertyValue
57 N90430995aa0d42d0b71b50bae28ce0df schema:name Springer Nature - SN SciGraph project
58 rdf:type schema:Organization
59 Na7cf78a6e80f4e8abb9f4fdbbb9743ff schema:name readcube_id
60 schema:value 28d5065b00ddb3e6f24e55dc6abb512f98429e7f76239783d7fe461ed756703f
61 rdf:type schema:PropertyValue
62 Nae34e0721f4a4e12a36aa765daf7f4b6 schema:issueNumber 4
63 rdf:type schema:PublicationIssue
64 Nee10197faf8e4de2b88630a15cf51b69 rdf:first sg:person.01033753364.95
65 rdf:rest rdf:nil
66 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
67 schema:name Biological Sciences
68 rdf:type schema:DefinedTerm
69 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
70 schema:name Biochemistry and Cell Biology
71 rdf:type schema:DefinedTerm
72 sg:journal.1083533 schema:issn 0175-7598
73 1432-0614
74 schema:name Applied Microbiology and Biotechnology
75 rdf:type schema:Periodical
76 sg:person.01033753364.95 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
77 schema:familyName Harder
78 schema:givenName Wim
79 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033753364.95
80 rdf:type schema:Person
81 sg:person.01322127264.74 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
82 schema:familyName Dijkhuizen
83 schema:givenName Lubbert
84 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322127264.74
85 rdf:type schema:Person
86 sg:pub.10.1007/bf00446560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033607761
87 https://doi.org/10.1007/bf00446560
88 rdf:type schema:CreativeWork
89 sg:pub.10.1007/bf00498724 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053723453
90 https://doi.org/10.1007/bf00498724
91 rdf:type schema:CreativeWork
92 sg:pub.10.1007/bf00505015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015922032
93 https://doi.org/10.1007/bf00505015
94 rdf:type schema:CreativeWork
95 sg:pub.10.1007/bf00510565 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034361920
96 https://doi.org/10.1007/bf00510565
97 rdf:type schema:CreativeWork
98 sg:pub.10.1007/bf00703041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021068553
99 https://doi.org/10.1007/bf00703041
100 rdf:type schema:CreativeWork
101 https://app.dimensions.ai/details/publication/pub.1075763875 schema:CreativeWork
102 https://doi.org/10.1002/bit.260130412 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051326196
103 rdf:type schema:CreativeWork
104 https://doi.org/10.1002/bit.260181111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050266771
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1002/bit.260211202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040101312
107 rdf:type schema:CreativeWork
108 https://doi.org/10.1002/bit.260220605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052647630
109 rdf:type schema:CreativeWork
110 https://doi.org/10.1002/bit.260230713 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046008729
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1002/bit.260230905 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053657003
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1016/0022-5193(73)90153-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021209388
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1016/s0076-6879(76)44031-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033803130
117 rdf:type schema:CreativeWork
118 https://www.grid.ac/institutes/grid.4830.f schema:alternateName University of Groningen
119 schema:name Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands
120 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...