A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass View Full Text


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Article Info

DATE

1978-03

AUTHORS

G. Braunegg, B. Sonnleitner, R. M. Lafferty

ABSTRACT

The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-β-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l. Only 4 h is required from sampling from the fermenter till completion of the PHB determination. More... »

PAGES

29-37

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00500854

DOI

http://dx.doi.org/10.1007/bf00500854

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040575757


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