Polymorphism of mitochondrial DNA in pigs based on restriction endonuclease cleavage patterns View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-02

AUTHORS

Tomomasa Watanabe, Yukimasa Hayashi, Nobuaki Ogasawara, Takeshi Tomoita

ABSTRACT

Restriction endonuclease cleavage patterns of mitochondrial DNA (mtDNA) of pigs and Japanese wild boars were analyzed using 17 enzymes which recognize six nucleotides. The map of cleavage sites was made by double-digestion methods. Polymophism of mtDNA was detected in the digestion by BglII, EcoRV, ScaI, and StuI. The restriction cleavage patterns were identical among the breeds of Landrace, Hampshire, Duroc I, and Large White I (A type). The patterns of Large White II were the same as those of Japanese wild boars (B type). A difference between the A type and the B type of mtDNA was found in the case of three restriction enzymes, BglII, ScaI, and StuI, and the nucleotide alterations between them were estimated as more than six. On the other hand, a difference between mtDNA from almost all pigs and mtDNA from Duroc II was detected using EcoRV. We suggest that the difference of mtDNA between the A type and the B type of mtDNA could result from the different origin of boars, that is, whether they were of European or Asian origin. More... »

PAGES

105-113

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00499116

DOI

http://dx.doi.org/10.1007/bf00499116

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032833535

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2986593


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