Denitrification of water using immobilized Pseudomonas denitrificans cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1980-12

AUTHORS

Inge Nilsson, Sten Ohlson, Lena Häggström, Nils Molin, Klaus Mosbach

ABSTRACT

Preparations of living Pseudomonas denitrificans cells immobilized in alginate gel were used in the denitrification of water. In the presence of an exogenous carbon source the entrapped microorganisms reduced nitrate and nitrite to gaseous products and to achieve complete reduction, carbon to nitrogen ratios of over two were required. The effects on denitrification of particle size and the number of bacteria in the gel were investigated. Apparent Km values for nitrate and nitrite reduction were calculated for free and immobilized cells. When the immobilized cells were incubated in nutrient media, an increase in reduction rate was observed and this was shown to be caused by the growth of cells within the gel particles. Immobilized P. denitrificans cells retained 75% of their initial nitrate reduction capacity after 21 days of storage at +4°C. The operational stability of the alginate-immobilized cells was studied both in batch and in a column which was operated continuously. A column (45 g of alginate-cell fibers in 80 ml) denitrified a high nitrate drinking water (100 mg NO3/l) with a rate of 300 ml of nitrate and nitrite free water/day/g of gel. The half life for nitrate reduction was estimated to be 30 days. More... »

PAGES

261-274

References to SciGraph publications

  • 1975-12. Immobilisation of microbial cells in polymeric matrices in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1969-12. Microorganisms, Alive and imprisoned in a Polymer Cage in NATURE
  • 1978-09. Degradation of β-lactam antibiotics by polyacrylamide-entrapped β-lactamase-producingE. coli cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1976-05. Microbial cells living immobilised on metal hydroxides in NATURE
  • 1978. Industrial applications of immobilized biocatalysts in ADVANCES IN BIOCHEMICAL ENGINEERING, VOLUME 10
  • 1977. The immobilization of whole cells in ADVANCES IN BIOCHEMICAL ENGINEERING, VOLUME 5
  • 1976-10. New approach to steroid conversion using activated immobilised microorganisms in NATURE
  • 1977-09. Screening of matrix suitable for immobilization of microbial cells in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1979-06. Steroid transformation by living cells immobilized in calcium alginate in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00498724

    DOI

    http://dx.doi.org/10.1007/bf00498724

    DIMENSIONS

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