Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-12

AUTHORS

Horst W. Doelle

ABSTRACT

Glucokinase (EC 2.7.1.2) exhibits two pH optima (pH 7.0 and 8.2), gives hyperbolic saturation curves and reacts equally with ATP, UTP, GTP, ITP and CTP. Inhibition occurs with high concentrations of these nucleotides and in addition with ADP, AMP and glucose 6-phosphate. No inhibition was observed with sucrose, glucose, fructose (11 mM), ethanol (542 mM), mannose, ribose, galactose, deoxy-glucose, lactose and gluconate and no reaction except with glucose. Fructokinase (EC 2.7.1.4) exhibits one pH optimum (7.4), gives hyperbolic saturation curves and is highly specific towards ATP and fructose. Mannose, glucose, GTP and CTP do not react. Inhibition occurs with glucose, glucose 6-phosphate and mildly fructose 6-phosphate. ATP at high concentrations gives slight inhibition, ADP and AMP show differential effects, whereas all other above mentioned compounds do not inhibit. Regulatory mechanisms for sucrose, glucose and fructose metabolism are discussed. More... »

PAGES

241-246

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00498471

DOI

http://dx.doi.org/10.1007/bf00498471

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047449812


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