Ontology type: schema:ScholarlyArticle
1987-03
AUTHORSA. C. Douma, M. Veenhuis, G. J. Sulter, W. Harder
ABSTRACTThe association of an ATPase with the yeast peroxisomal membrane was established by both biochemical and cytochemical procedures. Peroxisomes were purified from protoplast homogenates of the methanol-grown yeast Hansenula polymorpha by differential and sucrose gradient centrifugation. Biochemical analysis revealed that ATPase activity was associated with the peroxisomal peak fractions which were identified on the basis of alcohol oxidase and catalase activity. The properties of this ATPase closely resembled those of the mitochondrial ATPase of this yeast. The enzyme was Mg2+-dependent, had a pH optimum of approximately 8.5 and was sensitive to N,N'-dicyclohexylcarbodiimide (DCCD), oligomycin and azide, but not to vanadate. A major difference was the apparent Km for ATP which was 4-6 mM for the peroxisomal ATPase compared to 0.6-0.9 mM for the mitochondrial enzyme. Cytochemical experiments indicated that the peroxisomal ATPase was associated with the membranes surrounding these organelles. After incubations with CeCl3 and ATP specific reaction products were localized on the peroxisomal membrane, both when unfixed isolated peroxisomes or formaldehyde-fixed protoplasts were used. This staining was strictly ATP-dependent; in controls performed in the absence of substrate, in the presence of glycerol 2-phosphate instead of ATP, or in the presence of DCCD, staining was invariably absent. Similar staining patterns were observed in subcellular fractions and protoplasts of Candida utilis and Trichosporon cutaneum X4, grown in the presence of ethanol/ethylamine or ethylamine, respectively. More... »
PAGES42-47
http://scigraph.springernature.com/pub.10.1007/bf00492903
DOIhttp://dx.doi.org/10.1007/bf00492903
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1014972633
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/2883951
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Candida",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hydrogen-Ion Concentration",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Intracellular Membranes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microbodies",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Microscopy, Electron",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pichia",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Proton-Translocating ATPases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protoplasts",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Saccharomycetales",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Trichosporon",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Groningen",
"id": "https://www.grid.ac/institutes/grid.4830.f",
"name": [
"Department of Microbiology Biological Centre, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
],
"type": "Organization"
},
"familyName": "Douma",
"givenName": "A. C.",
"id": "sg:person.0617111667.86",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617111667.86"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Groningen",
"id": "https://www.grid.ac/institutes/grid.4830.f",
"name": [
"Laboratory for Electron Microscopy, Biological Centre, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
],
"type": "Organization"
},
"familyName": "Veenhuis",
"givenName": "M.",
"id": "sg:person.0677675651.70",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0677675651.70"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Groningen",
"id": "https://www.grid.ac/institutes/grid.4830.f",
"name": [
"Department of Microbiology Biological Centre, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
],
"type": "Organization"
},
"familyName": "Sulter",
"givenName": "G. J.",
"id": "sg:person.01102631634.79",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102631634.79"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Groningen",
"id": "https://www.grid.ac/institutes/grid.4830.f",
"name": [
"Department of Microbiology Biological Centre, University of Groningen, Kerklaan 30, NL-9751 NN, Haren, The Netherlands"
],
"type": "Organization"
},
"familyName": "Harder",
"givenName": "W.",
"id": "sg:person.01033753364.95",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033753364.95"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1042/bj2100685",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000404103"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj2100685",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000404103"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0076-6879(74)32039-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1008435695"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00415606",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010112168",
"https://doi.org/10.1007/bf00415606"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00409104",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015979343",
"https://doi.org/10.1007/bf00409104"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00409104",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015979343",
"https://doi.org/10.1007/bf00409104"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0076-6879(55)01006-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017768897"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1432-1033.1977.tb11812.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018482044"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00492902",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018669559",
"https://doi.org/10.1007/bf00492902"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00492902",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018669559",
"https://doi.org/10.1007/bf00492902"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.cb.01.110185.002421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019313372"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00402303",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024515034",
"https://doi.org/10.1007/bf00402303"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00402303",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024515034",
"https://doi.org/10.1007/bf00402303"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00411242",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024852105",
"https://doi.org/10.1007/bf00411242"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00411242",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024852105",
"https://doi.org/10.1007/bf00411242"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1574-6968.1980.tb05654.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033523377"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1574-6968.1980.tb05654.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033523377"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00446560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033607761",
"https://doi.org/10.1007/bf00446560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00446560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033607761",
"https://doi.org/10.1007/bf00446560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00413025",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042777316",
"https://doi.org/10.1007/bf00413025"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00428343",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048820816",
"https://doi.org/10.1007/bf00428343"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0304-4157(84)90004-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052991319"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0304-4157(84)90004-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052991319"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076956918",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079449557",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080073498",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1081575256",
"type": "CreativeWork"
}
],
"datePublished": "1987-03",
"datePublishedReg": "1987-03-01",
"description": "The association of an ATPase with the yeast peroxisomal membrane was established by both biochemical and cytochemical procedures. Peroxisomes were purified from protoplast homogenates of the methanol-grown yeast Hansenula polymorpha by differential and sucrose gradient centrifugation. Biochemical analysis revealed that ATPase activity was associated with the peroxisomal peak fractions which were identified on the basis of alcohol oxidase and catalase activity. The properties of this ATPase closely resembled those of the mitochondrial ATPase of this yeast. The enzyme was Mg2+-dependent, had a pH optimum of approximately 8.5 and was sensitive to N,N'-dicyclohexylcarbodiimide (DCCD), oligomycin and azide, but not to vanadate. A major difference was the apparent Km for ATP which was 4-6 mM for the peroxisomal ATPase compared to 0.6-0.9 mM for the mitochondrial enzyme. Cytochemical experiments indicated that the peroxisomal ATPase was associated with the membranes surrounding these organelles. After incubations with CeCl3 and ATP specific reaction products were localized on the peroxisomal membrane, both when unfixed isolated peroxisomes or formaldehyde-fixed protoplasts were used. This staining was strictly ATP-dependent; in controls performed in the absence of substrate, in the presence of glycerol 2-phosphate instead of ATP, or in the presence of DCCD, staining was invariably absent. Similar staining patterns were observed in subcellular fractions and protoplasts of Candida utilis and Trichosporon cutaneum X4, grown in the presence of ethanol/ethylamine or ethylamine, respectively.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00492903",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1018945",
"issn": [
"0302-8933",
"1432-072X"
],
"name": "Archives of Microbiology",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "147"
}
],
"name": "A proton-translocating adenosine triphosphatase is associated with the peroxisomal membrane of yeasts",
"pagination": "42-47",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"4755d6be5abffd2d0537896fe4cd14c71e9ac3eac3f2fc335e04f6e4eab7103d"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"2883951"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"0410427"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00492903"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1014972633"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00492903",
"https://app.dimensions.ai/details/publication/pub.1014972633"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:54",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130811_00000001.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00492903"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00492903'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00492903'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00492903'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00492903'
This table displays all metadata directly associated to this object as RDF triples.
195 TRIPLES
21 PREDICATES
59 URIs
31 LITERALS
19 BLANK NODES