A 31P NMR study of the internal pH of yeast peroxisomes View Full Text


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Article Info

DATE

1987-03

AUTHORS

K. Nicolay, M. Veenhuis, A. C. Douma, W. Harder

ABSTRACT

The internal pH of peroxisomes in the yeasts Hansenula polymorpha, Candida utilis and Trichosporon cutaneum X4 was estimated by 31P nuclear magnetic resonance (NMR) spectroscopy. 31P NMR spectra of suspensions of intact cells of these yeasts, grown under conditions of extensive peroxisomal proliferation, displayed two prominent Pi-peaks at different chemical shift positions. In control cells grown on glucose, which contain very few peroxisomes, only a single peak was observed. This latter peak, which was detected under all growth conditions, was assigned to cytosolic Pi at pH 7.1. The additional peak present in spectra of peroxisome-containing cells, reflected Pi at a considerably lower pH of approximately 5.8-6.0. Pi at a considerably lower pH of approximately 5.8-6.0. Experiments with the protonophore carbonyl cyanide m-chlorophenylhydrazon (CCCP) and the ionophores valinomycin and nigericin revealed that separation of the two Pi-peaks was caused by a pH-gradient across a membrane separating the two pools. Experiments with chloroquine confirmed the acidic nature of one of these pools. In a number of transfer experiments with the yeast H. polymorpha it was shown that the relative intensity of the Pi-signal at the low pH-position was correlated to the peroxisomal volume fraction. These results strongly suggest that this peak has to be assigned to Pi in peroxisomes, which therefore are acidic in nature. The presence of peroxisome-associated Pi was confirmed cytochemically. More... »

PAGES

37-41

References to SciGraph publications

  • 1983-07. In vivo 31P NMR studies on the role of the vacuole in phosphate metabolism in yeasts in ARCHIVES OF MICROBIOLOGY
  • 1983-10. Characterization of glyoxysomes in yeasts and their transformation into peroxisomes in response to changes in environmental conditions in ARCHIVES OF MICROBIOLOGY
  • 1985-12. Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha in ARCHIVES OF MICROBIOLOGY
  • 1979-02. Characterization of peroxisomes in glucose-grown Hansenula polymorpha and their development after the transfer of cells into methanol-containing media in ARCHIVES OF MICROBIOLOGY
  • 1987-03. A proton-translocating adenosine triphosphatase is associated with the peroxisomal membrane of yeasts in ARCHIVES OF MICROBIOLOGY
  • 1985-01. Biogenesis and metabolic significance of microbodies in urate-utilizing yeasts in ANTONIE VAN LEEUWENHOEK
  • 1985-06. The electrochemical H+ gradient in the yeastRhodotorula glutinis in JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
  • 1984-02. Effect of chloroquine on proteolytic processes and energy metabolism in yeast in ARCHIVES OF MICROBIOLOGY
  • 1986-06. Physiological role of microbodies in the yeast Trichosporon cutaneum during growth on ethylamine as the source of energy, carbon and nitrogen in ARCHIVES OF MICROBIOLOGY
  • 1982-12. Phosphorus-31 nuclear magnetic resonance studies of intracellular pH, phosphate compartmentation and phosphate transport in yeasts in ARCHIVES OF MICROBIOLOGY
  • 1983-06. Degradation and turnover of peroxisomes in the yeast Hansenula polymorpha induced by selective inactivation of peroxisomal enzymes in ARCHIVES OF MICROBIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00492902

    DOI

    http://dx.doi.org/10.1007/bf00492902

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1018669559

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/3579461


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