Oligo-riboprobes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-09

AUTHORS

P. Denny, Q. Hamid, J. E. Krause, J. M. Polak, S. Legon

ABSTRACT

In situ hybridisation detection of mRNAs using riboprobes has become a widely used technique. However, the identification of cells producing closely-related yet distinct mRNAs is difficult with the usual size probes. Moreover, it is not always easy to obtain the required cDNA essential for cRNA probe synthesis. To avoid these problems, we have used synthetic oligodeoxynucleotides to generate short, single stranded RNA probes ("oligo-riboprobes"). These probes can be labelled to very high (10(9) cpm/micrograms) specific activity and can be prepared for any published nucleotide sequence. We have used these probes to localise beta (preprotachykinin) PPT mRNA producing neurons in rat hypothalamus and bowel. The results were compared to that obtained with cRNA probes generated from beta preprotachykinin cDNA. More... »

PAGES

481-483

Journal

TITLE

Histochemistry and Cell Biology

ISSUE

5

VOLUME

89

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00492605

DOI

http://dx.doi.org/10.1007/bf00492605

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047496642

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3170268


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