Linear mitochondrial plasmid inBrassica has terminal protein View Full Text


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Article Info

DATE

1985-01

AUTHORS

L. Erickson, W. D. Beversdorf, K. P. Pauls

ABSTRACT

A linear 11.3 kb plasmid occurs in preparations of mitochondrial DNA from certainBrassica species and cultivars. Evidence that protein is associated with the ends of the plasmid was obtained in this study. Plasmid DNA ran as a distinct band during electrophoresis only if it had been treated with proteinase K. The hybridization patterns of clones from different regions of the plasmid to restriction digests of the protein-DNA complex revealed that the protein is associated with the end fragments of the plasmid. Exonuclease treatments indicated that the 5′ ends of the plasmid DNA molecule are blocked but that the 3′ ends are free. More... »

PAGES

679-682

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00449821

DOI

http://dx.doi.org/10.1007/bf00449821

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016191953


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